Details

Autor(en) / Beteiligte

• Sakai, S.
• Kim, J.
• Hexig, B.
• Okahata, Y.
• Cho, C.S.
• Akaike, T.

Titel

Adsorption behaviors of recombinant E-cadherin-IgG Fc fusion protein on polystyrene surface

Ist Teil von

• Colloids and surfaces, B, Biointerfaces, 2012-06, Vol.94, p.192-198

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2012

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• [Display omitted] ► E-cadherin-IgG Fc (E-cad-Fc) was stably adsorbed on polystyrene (PS) surface as a Langmuir-type in a monolayer. ► E-cadherin homophilic adhesion among E-cadherins during adsorption of E-cad-Fc is involved. ► Deglycosylation of the E-cadherin in the E-cad-Fc did not affect adsorption of E-cad-Fc on the PS surface. Adsorption behaviors of recombinant E-cadherin-IgG Fc (E-cad-Fc) fusion protein and mutated E-cad-Fcs on the polystyrene (PS) surface were investigated using a 27MHz quartz-crystal microbalance (QCM) and ELISA. The amount of adsorbed E-cad-Fc on PS surface was increased with an increase of E-cad-Fc concentration as a Langmuir-type in a monolayer. Adsorbed E-cad-Fc on PS surface was stable even after washing if calcium ions are absent in the washing solution due to the calcium ion dependence in the adsorption. E-cadherin homophilic adhesion among E-cadherins during adsorption of E-cad-Fc was involved. Deglycosylation of the E-cad in the E-cad-Fc did not affect adsorption of E-cad-Fc on the PS surface although deglycosylation of the E-cad in the E-cad-Fc enhanced cell adhesion compared with E-cad-Fc.