Cell stem cell, 2010-02, Vol.6 (2), p.175-181
2010
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Details
- Autor(en) / Beteiligte
- Titel
- Spindle Orientation Bias in Gut Epithelial Stem Cell Compartments Is Lost in Precancerous Tissue
- Ist Teil von
- Cell stem cell, 2010-02, Vol.6 (2), p.175-181
- Ort / Verlag
- Cambridge, MA: Elsevier Inc
- Erscheinungsjahr
- 2010
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- The importance of asymmetric divisions for stem cell function and maintenance is well established in the developing nervous system and the skin; however, its role in gut epithelium and its importance for tumorigenesis is still debated. We demonstrate alignment of mitotic spindles perpendicular to the apical surface specifically in the stem cell compartments of mouse and human intestine and colon. This orientation correlates with the asymmetric retention of label-retaining DNA. Both the preference for perpendicular spindle alignment and asymmetric label retention are lost in precancerous tissue heterozygous for the adenomatous polyposis coli tumor suppressor ( Apc). This loss correlates with cell shape changes specifically in the stem cell compartment. Our data suggest that loss of asymmetric division in stem cells might contribute to the oncogenic effect of Apc mutations in gut epithelium. ► Mitotic spindles in intestinal stem cells align perpendicularly ► In precancerous tissue, this spindle orientation bias is lost ► Spindle alignment correlates with segregation of unreplicated DNA ► Loss of unreplicated DNA occurs more quickly in precancerous tissue
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1934-5909eISSN: 1875-9777DOI: 10.1016/j.stem.2009.12.007Titel-ID: cdi_proquest_miscellaneous_954629646
- Format
- –
- Schlagworte
- Adenomatous Polyposis Coli Protein - genetics, Adenomatous Polyposis Coli Protein - metabolism, Animals, Biological and medical sciences, Cell differentiation, maturation, development, hematopoiesis, Cell Division, Cell physiology, Cell Shape, Colon - cytology, Colon - metabolism, Epithelial Cells - cytology, Epithelial Cells - metabolism, Fundamental and applied biological sciences. Psychology, Humans, Intestines - cytology, Intestines - metabolism, Mice, Mice, Inbred C57BL, Molecular and cellular biology, Mutation, Precancerous Conditions - genetics, Precancerous Conditions - metabolism, Precancerous Conditions - pathology, Spindle Apparatus - metabolism, Stem Cells - cytology, Stem Cells - metabolism, STEMCELL