Brain research, 2010-10, Vol.1358, p.239-247
2010
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- Autor(en) / Beteiligte
- Titel
- Protective effect of 3-butyl-6-bromo-1(3H)-isobenzofuranone on hydrogen peroxide-induced damage in PC12 cells
- Ist Teil von
- Brain research, 2010-10, Vol.1358, p.239-247
- Ort / Verlag
- Amsterdam: Elsevier B.V
- Erscheinungsjahr
- 2010
- Quelle
- Elsevier ScienceDirect Journals
- Beschreibungen/Notizen
- Abstract Oxidative stress, an imbalance toward the prooxidant side of the prooxidant/antioxidant homeostasis, occurs in several brain neurodegenerative disorders. The protective effect of 3-butyl-6-bromo-1(3H)-isobenzofuranone (Br-NBP), a derivative compound of l-3-n-butylphthalide (NBP), on the hydrogen peroxide(H2 O2 )- induced oxidative damage was investigated in PC12 cells. Following exposure of the cells to H2 O2 , there was a significant reduction in cell survival,activities of glutathione peroxidase (GSH-px) and mitochondria membrane potential(MMP), in contrast, the increased levels in the leakage of lactate dehydrogenase (LDH), malondialdehyde (MDA) contents, nitric oxide (NO) productions, intracellular reactive oxygen species (ROS), intracellular Ca2+ concentration ([Ca2+ ]i ), as well as cell apoptosis were observed. However, pretreatment of cells with Br-NBP prior to H2 O2 exposure attenuated all the changes mentioned above in a concentration-dependent manner except no effect on activities of GSH-px. Br-NBP exhibited the protective effect against H2 O2 -induced cytotoxicity in PC12 cells, suggesting that the compound may be a potential therapeutic agent for diseases induced by oxidative damage.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0006-8993eISSN: 1872-6240DOI: 10.1016/j.brainres.2010.08.043Titel-ID: cdi_proquest_miscellaneous_954581186
- Format
- –
- Schlagworte
- 3-butyl-6-bromo-1(3H)-isobenzofuranone, [Ca2+]i, Animals, Antioxidants, Apoptosis, Apoptosis - drug effects, Benzofurans - pharmacology, Biological and medical sciences, Calcium - metabolism, Cell Survival - drug effects, Drug Interactions, Fundamental and applied biological sciences. Psychology, Glutathione Peroxidase - metabolism, Hydrogen Peroxide - toxicity, Isolated neuron and nerve. Neuroglia, L-Lactate Dehydrogenase - metabolism, Malondialdehyde - metabolism, Membrane Potential, Mitochondrial - drug effects, MMP, Neurology, Neuroprotective Agents - pharmacology, Oxidants - toxicity, Oxidative stress, Oxidative Stress - drug effects, PC12 Cells - drug effects, Rats, Reactive Oxygen Species - metabolism, ROS, Vertebrates: nervous system and sense organs