FEBS letters, 2010-07, Vol.584 (13), p.2896-2900
2010
Details
- Autor(en) / Beteiligte
- Titel
- A lipocalin sequence signature modulates cell survival
- Ist Teil von
- FEBS letters, 2010-07, Vol.584 (13), p.2896-2900
- Ort / Verlag
- England: Elsevier B.V
- Erscheinungsjahr
- 2010
- Link zum Volltext
- Quelle
- Wiley Online Library Journals Frontfile Complete
- Beschreibungen/Notizen
- Lipocalins are β-barrel proteins, which share three conserved motifs in their amino acid sequence. In this study, we identified by a peptide mapping approach, a seven-amino acid sequence related to one of these motifs (motif 2) that modulates cell survival. A synthetic peptide based on an insect lipocalin displayed cytoprotective activity in serum-deprived endothelial cells and leucocytes. This activity was dependent on nitric oxide synthase. This sequence was found within several lipocalins, including apolipoprotein D, retinol binding protein, lipocalin-type prostaglandin D synthase, and many unknown proteins, suggesting that it is a sequence signature and a lipocalin conserved property.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0014-5793eISSN: 1873-3468DOI: 10.1016/j.febslet.2010.05.008Titel-ID: cdi_proquest_miscellaneous_954519410
- Format
- –
- Schlagworte
- 3-(4,5-dimethylthiazol-2-methyl)-2,5-diphenyltetrazolium bromide, Amino Acid Motifs, Animals, Apoptosis, Apoptosis - drug effects, Cell survival, Cell Survival - drug effects, Cells, Cultured, Endothelial Cells - cytology, Endothelial Cells - drug effects, FBS, fetal bovine serum, human umbilical vein endothelial cells, Humans, HUVECs, l-NAME, Lipocalin, Lipocalins - chemistry, Lonomia obliqua prothrombin activator protease, Lopap, MTT, nitric oxide, Nitric Oxide Synthase - metabolism, Nω-nitro-l-arginine methyl ester, Peptides - chemical synthesis, Peptides - chemistry, Peptides - pharmacology, Rats, relative surface accessibility, Roswell Park Memorial Institute 1640 medium, RPMI, RSA, Sequence motif