Journal of industrial microbiology & biotechnology, 2012-03, Vol.39 (3), p.459-469
2012
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- Autor(en) / Beteiligte
- Titel
- Production of polyhydroxyalkanoates by Burkholderia cepacia ATCC 17759 using a detoxified sugar maple hemicellulosic hydrolysate
- Ist Teil von
- Journal of industrial microbiology & biotechnology, 2012-03, Vol.39 (3), p.459-469
- Ort / Verlag
- Berlin/Heidelberg: Springer-Verlag
- Erscheinungsjahr
- 2012
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Sugar maple hemicellulosic hydrolysate containing 71.9 g/l of xylose was used as an inexpensive feedstock to produce polyhydroxyalkanoates (PHAs) by Burkholderia cepacia ATCC 17759. Several inhibitory compounds present in wood hydrolysate were analyzed for effects on cell growth and PHA production with strong inhibition observed at concentrations of 1 g/l furfural, 2 g/l vanillin, 7 g/l levulinic acid, and 1 M acetic acid. Gradual catabolism of lower concentrations of these inhibitors was observed in this study. To increase the fermentability of wood hydrolysate, several detoxification methods were tested. Overliming combined with low-temperature sterilization resulted in the highest removal of total inhibitory phenolics (65%). A fed-batch fermentation exhibited maximum PHA production after 96 h (8.72 g PHA/L broth and 51.4% of dry cell weight). Compositional analysis by NMR and physical–chemical characterization showed that PHA produced from wood hydrolysate was composed of polyhydroxybutyrate (PHB) with a molecular mass (M N) of 450.8 kDa, a melting temperature (T m) of 174.4°C, a glass transition temperature (T g) of 7.31°C, and a decomposition temperature (T decomp) of 268.6°C.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1367-5435eISSN: 1476-5535DOI: 10.1007/s10295-011-1040-6Titel-ID: cdi_proquest_miscellaneous_926892340
- Format
- –
- Schlagworte
- Acer - metabolism, Acer saccharum, Acer saccharum subsp. saccharum, Acetates - metabolism, Acetic acid, Acetic Acid - metabolism, Analysis, Bacteria, batch fermentation, Benzaldehydes - metabolism, Biochemistry, Biodegradation, Environmental, Bioinformatics, Biological and medical sciences, Biomedical and Life Sciences, Bioreactors, Biotechnology, Burkholderia cepacia, Burkholderia cepacia - genetics, Burkholderia cepacia - metabolism, Carbon, Cell Culture and Bioengineering, Cell growth, Cellulose - chemistry, Cellulose - metabolism, Charcoal, Chemical synthesis, Chromatography, Detoxification, Environmental science, feedstocks, Fermentation, Forestry, Fundamental and applied biological sciences. Psychology, Furaldehyde - metabolism, furfural, Genetic Engineering, glass transition temperature, Inorganic Chemistry, levulinic acid, Levulinic Acids - metabolism, Life Sciences, Lignin, Low temperature, melting point, Methods. Procedures. Technologies, Microbial engineering. Fermentation and microbial culture technology, Microbiology, Microorganisms, Molecular Weight, Nitrogen, nuclear magnetic resonance spectroscopy, phenolic compounds, Phenols, Polyhydroxyalkanoates, Polyhydroxyalkanoates - biosynthesis, Polyhydroxybutyrate, Protein Hydrolysates - chemistry, Protein Hydrolysates - metabolism, Sterilization, Studies, Sugar, Sulfuric acid, Transition temperatures, vanillin, wood, Wood - metabolism, xylose, Xylose - metabolism