Details

Autor(en) / Beteiligte

• Richetti, Aline
• Munaretto, Cristiane B.
• Lerin, Lindomar A.
• Batistella, Luciane
• Vladimir Oliveira, J.
• Dallago, Rogério M.
• Astolfi, Viviane
• Luccio, Marco Di
• Mazutti, Márcio A.
• de Oliveira, Débora
• Treichel, Helen

Titel

Immobilization of inulinase from Kluyveromyces marxianus NRRL Y-7571 using modified sodium alginate beads

Ist Teil von

• Bioprocess and biosystems engineering, 2012-03, Vol.35 (3), p.383-388

Ort / Verlag

Berlin/Heidelberg: Springer-Verlag

Erscheinungsjahr

2012

Link zum Volltext

Quelle

SpringerLink (Online service)

Beschreibungen/Notizen

• An experimental design was carried out to evaluate the effect of the concentrations of sodium alginate, glutaraldehyde and activated coal on the immobilization of inulinase from Kluyveromyces marxianus NRRL Y-7571. The experimental condition of 20 g/L of sodium alginate, 50 mL/L of glutaraldehyde and 30 g/L of activated coal led to the highest specific activity (2,063.5 U/mg of protein), corresponding to an enhancement of about 26 times compared to the activity of the free enzyme (79.1 U/mg of protein). The effect of pH and temperature on the immobilized enzyme activity was also evaluated, showing optimal activities at pH of 5.5 and 55 °C. The study of storage of immobilized inulinase in different temperatures showed that the extract kept its initial activity after 43 days of storage at 40 and 50 °C and after 138 days of storage either at 4 or 25 °C.