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Objective To determine the spinal innervation and neuronal connections is important for studying renal metabolic responses. Materials and Methods In this study, the spinal cords of 10 adult male C57BL/6J strain mice were mapped retrograde using injections of pseudorabies virus (PRV)-614. The virus, injected into the kidney, was specifically transported to the spinal cord. Results At 5 days after injection of the PRV-614, PRV-614–positive cells were found in the intermediolateral cell column, the intercalates nucleus, or the central autonomic nucleus of spinal cord segments T4-L1, and most PRV-614–labeled cells were found in the T9 segment. Conclusion Our results revealed neuroanatomical circuits between kidney and the spinal intermediolateral cell column neurons.