Details

Autor(en) / Beteiligte

• Sakagami, Hiroshi
• Iwamoto, Shoko
• Matsuta, Tomohiko
• Satoh, Kazue
• Shimada, Chiyako
• Kanamoto, Taisei
• Terakubo, Shigemi
• Nakashima, Hideki
• Morita, Yurika
• Ohkubo, Atsuko
• Tsuda, Tadashi
• Sunaga, Katsuyoshi
• Kitajima, Madoka
• Oizumi, Hiroshi
• Oizumi, Takaaki

Titel

Comparative study of biological activity of three commercial products of Sasa senanensis Rehder leaf extract

Ist Teil von

• In vivo (Athens), 2012-03, Vol.26 (2), p.259-264

Ort / Verlag

Greece

Erscheinungsjahr

2012

Quelle

MEDLINE

Beschreibungen/Notizen

• We have previously reported that alkaline extract of Sasa senanensis leaves (SE) has several biological activities characteristic of lignin-carbohydrate complex (LCC). In the present study, we compared the biological activity of three commercially available products of SE (products A, B and C). Cell viability of mock-infected, HIV-infected, UV-irradiated cells was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method. Radical intensity was determined by electron spin resonance spectroscopy. Cytochrome P-450 (CYP)3A4 activity was measured by β-hydroxylation of testosterone in human recombinant CYP3A4. Product A is a pure SE that contains Fe(II)-chlorophyllin, whereas products B and C contain Cu(II)-chlorophyllin and less LCC. Product C is supplemented with ginseng and pine (Pinus densiflora) leaf extracts. Product A exhibited 5-fold higher anti-HIV, 4-fold higher anti-UV, 5-fold higher hydroxyl radical-scavenging, and 3-fold lower CYP3A4 inhibitory activities as compared to those of product B, and 5-fold higher, 1.5-fold higher, comparable, and 7-fold lower activities, respectively, as compared to those of product C. The present study demonstrates for the first time the superiority of product A over products B and C, suggesting the beneficial role of LCC and Fe(II)-chlorophyllin.