Details

Autor(en) / Beteiligte

• Audunsdottir, Sigridur S.
• Magnadottir, Bergljot
• Gisladottir, Berglind
• Jonsson, Zophonias O.
• Bragason, Birkir Th

Titel

The acute phase response of cod ( Gadus morhua L.): Expression of immune response genes

Ist Teil von

• Fish & shellfish immunology, 2012-02, Vol.32 (2), p.360-367

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2012

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• An acute phase response (APR) was experimentally induced in Atlantic cod ( Gadus morhua L.) by intramuscular injection of turpentine oil. The change in the expression of immune related genes was monitored in the anterior kidney and the spleen over a period of 7 days. The genes examined were two types of pentraxins, apolipoprotein A1 (ApoA-I), the complement component C3, interleukin-1β (IL-1β), transferrin, cathelicidin, and hepcidin. All genes were constitutively expressed in both organs and their expression amplified by the turpentine injection. A pattern of response was observed both with respect to the organ preference and to the timing of a maximum response. The increased gene expression of the pentraxins, ApoA-I and C3 was restricted to the anterior kidney, the gene expression of IL-1β, cathelicidin, and transferrin increased in both organs, while hepcidin gene expression was only significantly increased in the spleen. The pentraxins and ApoA-I appear to be early mediators of APR in cod, possibly stimulating C3 and IL-1β response, while the antimicrobial peptides may play a minor role. The increase in transferrin gene expression in both organs, and apparent indifference to cortisol release associated with the turpentine injection, suggests that this could be a typical acute phase protein in cod. ► An acute phase response (APR) was experimentally induced in Atlantic cod ( Gadus morhua L.). ► Changes in the expression of eight selected immune related genes were monitored by RTqPCR. ► The expression of all the genes studied was affected. ► A response pattern was observed with respect to organ preference and timing of maximum response. ► The pentraxins CRP-PI and CRP-PII and apolipoprotein A1 seem to be early mediators of APR in cod.