Details

Autor(en) / Beteiligte

• Wolfgang, William J
• Coorevits, An
• Cole, Jocelyn A
• De Vos, Paul
• Dickinson, Michelle C
• Hannett, George E
• Jose, Reashma
• Nazarian, Elizabeth J
• Schumann, Peter
• Van Landschoot, Anita
• Wirth, Samantha E
• Musser, Kimberlee A

Titel

Sporosarcina newyorkensis sp. nov. from clinical specimens and raw cow’s milk

Ist Teil von

• International journal of systematic and evolutionary microbiology, 2012-02, Vol.62 (Pt 2), p.322-329

Ort / Verlag

Reading: International Union of Microbiological Societies

Erscheinungsjahr

2012

Quelle

MEDLINE

Beschreibungen/Notizen

• Twelve independent isolates of a Gram-positive, endospore-forming rod were recovered from clinical specimens in New York State, USA, and from raw milk in Flanders, Belgium. The 16S rRNA gene sequences for all isolates were identical. The closest species with a validly published name, based on 16S rRNA gene sequence, is Sporosarcina koreensis (97.13 % similarity). DNA–DNA hybridization studies demonstrate that the new isolates belong to a species distinct from their nearest phylogenetic neighbours. The partial sequences of the 23S rRNA gene for the novel strains and their nearest neighbours also provide support for the novel species designation. Maximum-likelihood phylogenetic analysis of the 16S rRNA gene sequences confirmed that the new isolates are in the genus SPOROSARCINA: The predominant menaquinone is MK-7, the peptidoglycan has the type A4α L-Lys–Gly–D-Glu, and the polar lipids consist of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The predominant fatty acids are iso-C14 : 0, iso-C15 : 0 and anteiso-C15 : 0. In addition, biochemical and morphological analyses support designation of the twelve isolates as representatives of a single new species within the genus Sporosarcina, for which the name Sporosarcina newyorkensis sp. nov. (type strain 6062T = DSM 23544T = CCUG 59649T = LMG 26022T) is proposed.