Details

Autor(en) / Beteiligte

• Li, S
• Sun, L-J
• Li, X
• Xiong, R-Y
• Xu, Q-F
• Zhou, Y-J

Titel

Construction of yeast two-hybrid cDNA library of high-viruliferous (RSV-infected) populations of the small brown planthopper, Laodelphax striatellus (Hemiptera: Delphacidae)

Ist Teil von

• K'un ch'ung hsueh pao, 2011-11, Vol.54 (11), p.1324-1328

Erscheinungsjahr

2011

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• In order to research the interaction between the small brown planthopper (Laodelphax striatellus Fallen, SBPH) and rice stripe virus (RSV), yeast two-hybrid cDNA library of high-viruliferous (RSV-infected) SBPH populations was constructed. SBPH infected with RSV was maintained in our laboratory. The mRNA was isolated and purified from high-viruliferous populations, and used as the template to synthesize the double-strand cDNAs. The cDNAs were ligated with three-frame adapter and purified by the cDNA size fractionation columns. By using homologous recombination reaction, the cDNA entry library was prepared and then recombined into Gateway compatible vector pGADT7-DEST to construct yeast two-hybrid cDNA library. Detection of the library indicated that it contained 3.68 x 10 super(7) independent clones, and the titer of the amplified library was 2.62 x 10 super(10) cfu/mL. The recombination rate was above 95%, and the average size of inserts was above 1 kb in the cDNA library. The results demonstrate that the li