Virology (New York, N.Y.), 2012-02, Vol.423 (2), p.107-118
2012
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- Autor(en) / Beteiligte
- Titel
- Lymph nodes harbor viral reservoirs that cause rebound of plasma viremia in SIV-infected macaques upon cessation of combined antiretroviral therapy
- Ist Teil von
- Virology (New York, N.Y.), 2012-02, Vol.423 (2), p.107-118
- Ort / Verlag
- United States
- Erscheinungsjahr
- 2012
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Abstract Attempts to find a cure for HIV infection are hindered by the presence of viral reservoirs that resist highly active antiretroviral therapy. To identify the properties of these reservoirs, four SIV239-infected Rhesus macaques were treated with combined antiretroviral therapy (cART) for 1 year. While plasma viral RNA (vRNA) was effectively suppressed, a systemic analysis revealed that vRNA was distributed in the following order: lymphatic tissues > lungs and intestine > other tissues. Histochemistry yielded no cells with viral signals. To increase the chance of detection, two additional SIV-infected animals were treated and analyzed on Day 10 after the cessation of cART. These animals exhibited similar vRNA distribution patterns to the former animals, and immunohistochemistry revealed Nef-positive T lymphocytes predominantly in the follicles of mesenteric lymph nodes (MLNs). These data suggest that lymphatic tissues, including MLNs, contain major cellular reservoirs that cause rebound of plasma viremia upon cessation of therapy.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0042-6822eISSN: 1096-0341DOI: 10.1016/j.virol.2011.11.024Titel-ID: cdi_proquest_miscellaneous_918054401
- Format
- –
- Schlagworte
- Animals, Anti-HIV Agents - therapeutic use, CD4-Positive T-Lymphocytes - virology, Disease Models, Animal, Disease Reservoirs - virology, Drug Therapy, Combination, Female, HIV Infections - drug therapy, HIV Infections - immunology, HIV Infections - virology, HIV-1 - drug effects, HIV-1 - physiology, Human immunodeficiency virus, Humans, Infectious Disease, Lymph Nodes - virology, Macaca mulatta, Recurrence, Simian Acquired Immunodeficiency Syndrome - drug therapy, Simian Acquired Immunodeficiency Syndrome - immunology, Simian Acquired Immunodeficiency Syndrome - virology, Simian Immunodeficiency Virus - drug effects, Simian Immunodeficiency Virus - physiology, Virus Latency, Virus Replication - drug effects