Details

Autor(en) / Beteiligte

• Weiss, Thomas W.
• Arnesen, Harald
• Trøseid, Marius
• Kaun, Christoph
• Hjerkinn, Elsa M.
• Huber, Kurt
• Wojta, Johann
• Seljeflot, Ingebjorg

Titel

Adipose tissue expression of interleukin-18 mRNA is elevated in subjects with metabolic syndrome and independently associated with fasting glucose

Ist Teil von

• Wiener Klinische Wochenschrift, 2011-11, Vol.123 (21-22), p.650-654

Ort / Verlag

Vienna: Springer-Verlag

Erscheinungsjahr

2011

Link zum Volltext

Quelle

SpringerLink (Online service)

Beschreibungen/Notizen

• Summary BACKGROUND: The metabolic syndrome (MetS) is a cluster of risk factors that are highly associated with increased risk for cardiovascular disease (CVD). Increased serum levels of plasminogen activator inhibitor-1 (PAI-1), interleukin-6 (IL-6) and IL-18 have been reported to be associated with CVD. Recently, IL-18 has been shown to be predictive for cardiovascular events in subjects with MetS. We have investigated the expression of PAI-1, IL-6 and IL-18 in subcutaneous adipose tissue (AT) of subjects with ( n = 22) and without ( n = 36) MetS. Furthermore, we have analysed the expression of IL-18 in monocyte-derived macrophages (MDMs) in an in vitro model of hyperglycaemia. METHODS: We studied the expression of PAI-1, IL-6 and IL-18 in biopsies of subcutaneous adipose tissue using Real-time PCR. After isolation and cultivation of MDMs, expression of IL-18 was determined by Real-time PCR. RESULTS: Expression of IL-18 was increased in subcutaneous AT of subjects with MetS ( p < 0.05). Multivariate analysis revealed fasting plasma glucose to be the only MetS component being independently associated with expression of IL-18 in AT ( p < 0.05). Exposure to hyperglycaemia, increased in expression of IL-18 in MDMs ( p < 0.01). CONCLUSION: Our findings suggest that subjects with MetS have a particular inflammatory pattern in AT, possibly driven by fasting glucose. MDMs might - at least in part - be the cellular source of this increased expression. Together with recent reports, showing IL-18 to be predictive for cardiovascular events, our findings could provide the basis for further research of the role of IL-18 as a link and possible target in the association between MetS and atherosclerosis.