Details

Autor(en) / Beteiligte

• Wang, Li-Wei
• Xu, Bai-Ge
• Wang, Jia-Ying
• Su, Zhen-Zhu
• Lin, Fu-Cheng
• Zhang, Chu-Long
• Kubicek, Christian P.

Titel

Bioactive metabolites from Phoma species, an endophytic fungus from the Chinese medicinal plant Arisaema erubescens

Ist Teil von

• Applied microbiology and biotechnology, 2012-02, Vol.93 (3), p.1231-1239

Ort / Verlag

Berlin/Heidelberg: Springer-Verlag

Erscheinungsjahr

2012

Quelle

SpringerLink

Beschreibungen/Notizen

• Through bioassay-guided fractionation, the EtOAc extract of a culture broth of the endophytic fungus Phoma species ZJWCF006 in Arisaema erubescens afforded a new α-tetralone derivative, (3 S )-3,6,7-trihydroxy-α-tetralone ( 1 ), together with cercosporamide ( 2 ), β-sitosterol ( 3 ), and trichodermin ( 4 ). The structures of compounds were established on the basis of spectroscopic analyses. Compounds 1 , 2 , and 3 were obtained from Phoma species for the first time. Additionally, the compounds were subjected to bioactivity assays, including antimicrobial activity, against four plant pathogenic fungi ( Fusarium oxysporium , Rhizoctonia solani , Colletotrichum gloeosporioides , and Magnaporthe oryzae ) and two plant pathogenic bacteria ( Xanthomonas campestris and Xanthomonas oryzae ), as well as in vitro antitumor activities against HT-29, SMMC-772, MCF-7, HL-60, MGC80-3, and P388 cell lines. Compound 1 showed growth inhibition against F. oxysporium and R. solani with EC 50 values of 413.22 and 48.5 μg/mL, respectively. Additionally, compound 1 showed no cytotoxicity, whereas compound 2 exhibited cytotoxic activity against the six tumor cell lines tested, with IC 50 values of 9.3 ± 2.8, 27.87 ± 1.78, 48.79 ± 2.56, 37.57 ± 1.65, 27.83 ± 0.48, and 30.37 ± 0.28 μM, respectively. We conclude that endophytic Phoma are promising sources of natural bioactive and novel metabolites.