Oncogene, 2012-01, Vol.31 (3), p.293-305
2012
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Identification and angiogenic role of the novel tumor endothelial marker CLEC14A
- Ist Teil von
- Oncogene, 2012-01, Vol.31 (3), p.293-305
- Ort / Verlag
- London: Nature Publishing Group UK
- Erscheinungsjahr
- 2012
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Tumor endothelial markers (TEMs) that are highly expressed in human tumor vasculature compared with vasculature in normal tissue hold clear therapeutic potential. We report that the C-type lectin CLEC14A is a novel TEM. Immunohistochemical and immunofluorescence staining of tissue arrays has shown that CLEC14A is strongly expressed in tumor vasculature when compared with vessels in normal tissue. CLEC14A overexpression in tumor vessels was seen in a wide range of solid tumor types. Functional studies showed that CLEC14A induces filopodia and facilitates endothelial migration, tube formation and vascular development in zebrafish that is, CLEC14A regulates pro-angiogenic phenotypes. CLEC14A antisera inhibited cell migration and tube formation, suggesting that anti-CLEC14A antibodies may have anti-angiogenic activity. Finally, in endothelial cultures, expression of CLEC14A increased at low shear stress, and we hypothesize that low shear stress due to poor blood flow in the disorganized tumor vasculature induces expression of CLEC14A on tumor vessels and pro-angiogenic phenotypes.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0950-9232eISSN: 1476-5594DOI: 10.1038/onc.2011.233Titel-ID: cdi_proquest_miscellaneous_917158103
- Format
- –
- Schlagworte
- Angiogenesis, Animals, Antibodies, Antisera, Apoptosis, Biomarkers, Tumor - analysis, Biomarkers, Tumor - genetics, Biomarkers, Tumor - metabolism, Breast Neoplasms - blood supply, Breast Neoplasms - metabolism, Cancer cells, Carcinoma, Hepatocellular - blood supply, Carcinoma, Hepatocellular - metabolism, Carcinoma, Hepatocellular - pathology, Cell adhesion & migration, Cell Adhesion Molecules - genetics, Cell Adhesion Molecules - metabolism, Cell Biology, Cell culture, Cell Line, Tumor, Cell migration, Cell Movement, Danio rerio, Development, Endothelium, Vascular - metabolism, Female, Gene expression, Genetic aspects, Genetic markers, Genotype & phenotype, Growth, Human Genetics, Humans, Identification and classification, Immunofluorescence, Immunohistochemistry, Internal Medicine, Lectins, Lectins, C-Type - genetics, Lectins, C-Type - metabolism, Liver Neoplasms - blood supply, Liver Neoplasms - metabolism, Liver Neoplasms - pathology, Male, Mechanical stimuli, Medicine, Medicine & Public Health, Neovascularization, Neovascularization, Pathologic - genetics, Neovascularization, Pathologic - metabolism, Oncology, original-article, Ovarian Neoplasms - blood supply, Ovarian Neoplasms - metabolism, Prostatic Neoplasms - blood supply, Prostatic Neoplasms - metabolism, Pseudopodia, Pseudopodia - metabolism, Solid tumors, Tumors, Urinary Bladder Neoplasms - blood supply, Urinary Bladder Neoplasms - metabolism, Vascular endothelium, Zebrafish