Details

Autor(en) / Beteiligte

• Li, Mei-Ying
• Xu, Bi-Yu
• Liu, Ju-Hua
• Yang, Xiao-Liang
• Zhang, Jian-Bin
• Jia, Cai-Hong
• Ren, Li-Cheng
• Jin, Zhi-Qiang

Titel

Identification and expression analysis of four 14-3-3 genes during fruit ripening in banana (Musa acuminata L. AAA group, cv. Brazilian)

Ist Teil von

• Plant cell reports, 2012-02, Vol.31 (2), p.369-378

Ort / Verlag

Berlin/Heidelberg: Springer-Verlag

Erscheinungsjahr

2012

Quelle

SpringerLink

Beschreibungen/Notizen

• To investigate the regulation of 14-3-3 proteins in banana ( Musa acuminata L. AAA group, cv. Brazilian) fruit postharvest ripening, four cDNAs encoding 14-3-3 proteins were isolated from banana and designated as Ma - 14 - 3 - 3a , Ma - 14 - 3 - 3c , Ma - 14 - 3 - 3e , and Ma - 14 - 3 - 3i , respectively. Amino acid sequence alignment showed that the four 14-3-3 proteins shared a highly conserved core structure and variable C-terminal as well as N-terminal regions with 14-3-3 proteins from other plant species. Phylogenetic analysis revealed that the four 14-3-3 genes belong to the non-ε groups. They were differentially and specifically expressed in various tissues. Real-time RT-PCR analysis indicated that these four genes function differentially during banana fruit postharvest ripening. Three genes, Ma - 14 - 3 - 3a , Ma - 14 - 3 - 3c , and Ma - 14 - 3 - 3e , were significantly induced by exogenous ethylene treatment. However, gene function differed in naturally ripened fruits. Ethylene could induce Ma - 14 - 3 - 3c expression during postharvest ripening, but expression patterns of Ma - 14 - 3 - 3a and Ma - 14 - 3 - 3e suggest that these two genes appear to be involved in regulating ethylene biosynthesis during fruit ripening. No obvious relationship emerged between Ma - 14 - 3 - 3i expression in naturally ripened and 1-MCP (1-methylcyclopropene)-treated fruit groups during fruit ripening. These results indicate that the 14-3-3 proteins might be involved in various regulatory processes of banana fruit ripening. Further studies will mainly focus on revealing the detailed biological mechanisms of these four 14-3-3 genes in regulating banana fruit postharvest ripening.