Details

Autor(en) / Beteiligte

• Oezdemir, Rena F.
• Gaisa, Nadine T.
• Lindemann-Docter, Katharina
• Gostek, Sonja
• Weiskirchen, Ralf
• Ahrens, Maike
• Schwamborn, Kristina
• Stephan, Christian
• Pfister, David
• Heidenreich, Axel
• Knuechel, Ruth
• Henkel, Corinna

Titel

Proteomic tissue profiling for the improvement of grading of noninvasive papillary urothelial neoplasia

Ist Teil von

• Clinical biochemistry, 2012-01, Vol.45 (1-2), p.7-11

Ort / Verlag

Amsterdam: Elsevier Inc

Erscheinungsjahr

2012

Quelle

ScienceDirect

Beschreibungen/Notizen

• In 2004, a novel grading system for papillary non-invasive bladder cancer was introduced; low grade (LG) and high grade (HG) in lieu of the former G1, G2, G3. This change allowed for increased reproducibility as well as diminished interobserver variability in histopathological grading among individual pathologists. Matrix Assisted Laser Desorption/Ionization Time of Flight Imaging Mass Spectrometry (MALDI TOF IMS) was evaluated as an automatic and objective tool to assist grading of urothelial neoplasms and to facilitate accuracy. To separate G1 (LG, n=27) and G3 (HG, n=21) papillary tumors MALDI TOF IMS was performed using an appropriate algorithm. Thereafter, the automatic assignment of a separate G2 (n=31) group was completed. G1 (LG) and G3 (HG) tumors were separated with an overall cross validation of 97.18%. G2 tumors indicated a true positive rate of 78.3% for LG and 87.5% for HG, respectively. MALDI TOF IMS is a powerful support tool to ascertain pathological diagnosis/grading. ► We were able to grade papillary bladder cancer tumors in an automated and objective way. ► We classified the former G2 tumor grade into the LG or HG group (WHO classification from 2004) based on a SVM algorithm. ► We reached high sensitivity and specificity in reclassification of G2 tumors. ► MALDI imaging offers great potential in clinical practice to ascertain pathological diagnosis/grading.