Journal of proteomics, 2012-01, Vol.75 (3), p.978-992
2012
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- Autor(en) / Beteiligte
- Titel
- Proteomic analysis and protein carbonylation profile in trained and untrained rat muscles
- Ist Teil von
- Journal of proteomics, 2012-01, Vol.75 (3), p.978-992
- Ort / Verlag
- Kidlington: Elsevier B.V
- Erscheinungsjahr
- 2012
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Understanding the relationship between physical exercise, reactive oxygen species and skeletal muscle modification is important in order to better identify the benefits or the damages that appropriate or inappropriate exercise can induce. Unbalanced ROS levels can lead to oxidation of cellular macromolecules and a major class of protein oxidative modification is carbonylation. The aim of this investigation was to study muscle protein expression and carbonylation patterns in trained and untrained animal models. We analyzed two muscles characterized by different metabolisms: tibialis anterior and soleus. Whilst tibialis anterior is mostly composed of fast-twitch fibers, the soleus muscle is mostly composed of slow-twitch fibers. By a proteomic approach we identified 15 protein spots whose expression is influenced by training. Among them in tibialis anterior we observed a down-regulation of several glycolitic enzymes. Concerning carbonylation, we observed the existence of a high basal level of protein carbonylation. Although this level shows some variation among individual animals, several proteins (mostly involved in energy metabolism, muscle contraction, and stress response) appear carbonylated in all animals and in both types of skeletal muscle. Moreover we identified 13 spots whose carbonylation increases after training. Synthetic gels representing the carbonylation pattern of soleus (A) and tibialis anterior (B) muscles. These gels were composed with the Oxyblots images from all animals (untrained and trained) and show only the carbonylated spots present in all gels. Red circles indicates spots that increased or are carbonylated (empty circles) only after training. [Display omitted] ► Animal model is used to study training impact on protein expression and carbonylation. ► Fast-twitch fibers muscle shows a decreased expression of several glycolytic enzymes. ► 13 proteins show an increased carbonylation level after training. ► The basal muscle proteins carbonylation pattern is analyzed.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1874-3919eISSN: 1876-7737DOI: 10.1016/j.jprot.2011.10.017Titel-ID: cdi_proquest_miscellaneous_912797432
- Format
- –
- Schlagworte
- animal models, Animals, Biological and medical sciences, Carbonylation, Diverse techniques, energy metabolism, enzymes, exercise, Exercise training, Fundamental and applied biological sciences. Psychology, Male, Molecular and cellular biology, Muscle, muscle contraction, Muscle Fibers, Fast-Twitch - metabolism, Muscle Fibers, Slow-Twitch - metabolism, muscle protein, Muscle Proteins - metabolism, muscles, oxidation, Oxidative stress, Oxyblots, Physical Conditioning, Animal - physiology, Protein Carbonylation - physiology, Protein Processing, Post-Translational - physiology, protein synthesis, proteins, Proteome - metabolism, proteomics, Proteomics - methods, Rats, Rats, Sprague-Dawley, reactive oxygen species, skeletal muscle, stress response, Two dimensional gel electrophoresis