Journal of cellular biochemistry, 2012-01, Vol.113 (1), p.204-208
2012
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- Autor(en) / Beteiligte
- Titel
- Hedgehog signaling and osteoblast gene expression are regulated by purmorphamine in human mesenchymal stem cells
- Ist Teil von
- Journal of cellular biochemistry, 2012-01, Vol.113 (1), p.204-208
- Ort / Verlag
- Hoboken: Wiley Subscription Services, Inc., A Wiley Company
- Erscheinungsjahr
- 2012
- Quelle
- Wiley Online Library Journals Frontfile Complete
- Beschreibungen/Notizen
- Several biological events are controlled by Hedgehog (Hh) signaling, including osteoblast phenotype development. This study aimed at evaluating the gene expression profile of human mesenchymal stem cells (hMSCs) treated with the Hh agonist, purmorphamine, focusing on Hh signaling and osteoblast differentiation. hMSCs from bone marrow were cultured in non‐osteogenic medium with or without purmorphamine (2 µM) for periods of up to 14 days. Purmorphamine up‐regulated gene expression of the mediators of Hh pathway, SMO, PTCH1, GLI1, and GLI2. The activation of Hh pathway by purmorphamine increased the expression of several genes (e.g., RUNX2 and BMPs) related to osteogenesis. Our results indicated that purmorphamine triggers Hh signaling pathway in hMSCs, inducing an increase in the expression of a set of genes involved in the osteoblast differentiation program. Thus, we conclude that Hh is a crucial pathway in the commitment of undifferentiated cells to the osteoblast lineage. J. Cell. Biochem. 113: 204–208, 2012. © 2011 Wiley Periodicals, Inc.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0730-2312eISSN: 1097-4644DOI: 10.1002/jcb.23345Titel-ID: cdi_proquest_miscellaneous_912638730
- Format
- –
- Schlagworte
- Cell Differentiation - drug effects, Cell Differentiation - genetics, Cells, Cultured, Core Binding Factor Alpha 1 Subunit - metabolism, Gene expression, Gene Expression Profiling, Hedgehog, Hedgehog Proteins - genetics, Hedgehog Proteins - metabolism, Humans, Kruppel-Like Transcription Factors - metabolism, Mesenchymal stem cell, Mesenchymal Stromal Cells - cytology, Mesenchymal Stromal Cells - drug effects, Mesenchymal Stromal Cells - metabolism, Morpholines - pharmacology, Nuclear Proteins - metabolism, Osteoblast, Osteoblasts - cytology, Osteogenesis - genetics, Patched Receptors, Patched-1 Receptor, Purines - pharmacology, Purmorphamine, Receptors, Cell Surface - metabolism, Receptors, G-Protein-Coupled - metabolism, RNA, Messenger - metabolism, Signal Transduction - genetics, Smoothened Receptor, Transcription Factors - metabolism, Zinc Finger Protein GLI1, Zinc Finger Protein Gli2