Molecular and cellular endocrinology, 2012-02, Vol.349 (2), p.214-221
2012
Details
- Autor(en) / Beteiligte
- Titel
- Melatonin inhibits glucocorticoid-dependent GR–TIF2 interaction in newborn hamster kidney (BHK) cells
- Ist Teil von
- Molecular and cellular endocrinology, 2012-02, Vol.349 (2), p.214-221
- Ort / Verlag
- Ireland: Elsevier Ireland Ltd
- Erscheinungsjahr
- 2012
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- ► Mechanisms involved on the glucocorticoid–melatonin antagonism in BHK cells. ► Melatonin impairs GR interaction with TIF2 coactivator in BHK cells. ► Antiglucocorticoid effects of the methoxyindol luzindole. The antagonism exerted by melatonin on the glucocorticoid response has been well established, being strongly dependent on the cellular context. Previously, we found that melatonin inhibits glucocorticoid receptor (GR) dissociation from the chaperone hetero-complex and nuclear translocation on mouse thymocytes. Here, by performing confocal fluorescence microscopy and the Number and Brightness assay we show that in newborn hamster kidney cells (BHK21) melatonin neither affects GR nuclear translocation nor GR homodimerization. Instead, co-immunoprecipitation studies suggest that physiological concentrations of melatonin impair GR interaction with the transcriptional intermediary factor 2 (TIF2). This melatonin effect was not blocked by the MT1/MT2 receptor antagonist luzindole. Curiously, luzindole behaved as an antiglucocorticoid per se by impairing the glucocorticoid-dependent MMTV-driven gene expression affecting neither GR translocation nor GR–TIF2 interaction.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0303-7207eISSN: 1872-8057DOI: 10.1016/j.mce.2011.10.026Titel-ID: cdi_proquest_miscellaneous_912271398
- Format
- –
- Schlagworte
- Active Transport, Cell Nucleus, Animals, Antagonism, antagonists, Cell Line, Cellular, Confocal, Cricetinae, Cyclic AMP - metabolism, Dexamethasone - pharmacology, Dimerization, dissociation, fluorescence microscopy, gene expression, Gene Expression - drug effects, glucocorticoid receptors, Glucocorticoids, Glucocorticoids - pharmacology, Hamsters, Immunoprecipitation, kidney cells, Kidneys, Luzindole, Mammary Tumor Virus, Mouse, Melatonin, Melatonin - pharmacology, mice, Microscopy, Microscopy, Fluorescence, neonates, Nuclear Receptor Coactivator 2 - genetics, Nuclear Receptor Coactivator 2 - metabolism, Receptors, Receptors, Glucocorticoid - genetics, Receptors, Glucocorticoid - metabolism, Receptors, Melatonin - antagonists & inhibitors, Receptors, Melatonin - genetics, Receptors, Melatonin - metabolism, Signal Transduction, thymocytes, TIF2, transcription (genetics), Tryptamines - pharmacology