Details

Autor(en) / Beteiligte

• Burgard, Marianne, MD
• Blanche, Stéphane, MD
• Jasseron, Carine, MS
• Descamps, Philippe, MD
• Allemon, Marie-Christine, MD
• Ciraru-Vigneron, Nicole, MD
• Floch, Corinne, MD
• Heller-Roussin, Brigitte, MD
• Lachassinne, Eric, MD
• Mazy, Fabienne, MD
• Warszawski, Josiane, MD, PhD
• Rouzioux, Christine, PharmD, PhD

Titel

Performance of HIV-1 DNA or HIV-1 RNA Tests for Early Diagnosis of Perinatal HIV-1 Infection during Anti-Retroviral Prophylaxis

Ist Teil von

• The Journal of pediatrics, 2012, Vol.160 (1), p.60-66.e1

Ort / Verlag

Maryland Heights, MO: Elsevier Inc

Erscheinungsjahr

2012

Quelle

MEDLINE

Beschreibungen/Notizen

• Objective To compare performance of testing for human immunodeficiency virus (HIV)-1 DNA and HIV-1 RNA for diagnosis of HIV-1 infection in infants receiving preventive antiretroviral therapy. Study design This substudy of the French multicenter prospective cohort of neonates born to HIV-infected mothers, included 1567 infants tested for HIV with polymerase chain reaction (PCR) in a single laboratory, receiving post-natal prophylaxis, not breastfed, and having simultaneous HIV-1 DNA and RNA results before 45 days. The performance of PCR was assessed in reference to the 6-month HIV-1 RNA result. Results Specificity of both HIV-1 RNA and HIV-1 DNA PCR was 100% at all ages (except 99.8% for DNA at birth); sensitivity was 58% (RNA) and 55% (DNA) at birth, and 89% at 1 month, 100% at 3 months for both, and 100% at 6 months (DNA). Concordance between HIV-1 DNA and RNA results was 0.78 and 0.81 (Kappa) at birth and 1 month and 100% at 3 and 6 months. Type of maternal and neonatal prophylaxis had no effect on sensitivity, but influenced viral load. Conclusion The performances of testing for HIV-1 DNA and RNA were similar with 100% sensitivity at 3 months. At 1 month during prophylaxis, 11% of infected children had negative PCR results.