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Molecular characterization of Cryptosporidium spp. in dairy calves from the state of São Paulo, Brazil
Ist Teil von
Parasitology research (1987), 2011-09, Vol.109 (3), p.949-951
Ort / Verlag
Berlin/Heidelberg: Springer-Verlag
Erscheinungsjahr
2011
Quelle
MEDLINE
Beschreibungen/Notizen
Cryptosporidiosis is a common protozoan disease observed in a wide range of vertebrate hosts, including ruminants. Cattle can be a potential reservoir of
Cryptosporidium
spp., leading to environmental contamination with oocysts of zoonotic species. The molecular characterization of
Cryptosporidium
spp. isolated from cattle from the state of São Paulo, Brazil, was accomplished using nested polymerase chain reaction for amplification of fragments of the 18S rRNA gene and the glycoprotein GP60 gene, following sequencing of amplified fragments. Positivity for
Cryptosporidium
was found in 10.7% (21/196) of the samples. Four species of
Cryptosporidium
were identified:
C. andersoni
,
C. bovis
,
C. parvum
subtype IIaA15G2R1, and
C. ryanae
. To the best of our knowledge, this is the first report of infection by
C. ryanae
and
C. parvum
IIaA15G2R1 in cattle from Brazil.