Science (American Association for the Advancement of Science), 2011-01, Vol.331 (6016), p.473-477
2011
Details
- Autor(en) / Beteiligte
- Titel
- Intramembrane Cleavage of AMA1 Triggers Toxoplasma to Switch from an Invasive to a Replicative Mode
- Ist Teil von
- Science (American Association for the Advancement of Science), 2011-01, Vol.331 (6016), p.473-477
- Ort / Verlag
- Washington, DC: American Association for the Advancement of Science
- Erscheinungsjahr
- 2011
- Link zum Volltext
- Quelle
- American Association for the Advancement of Science
- Beschreibungen/Notizen
- Apicomplexan parasites invade host cells and immediately initiate cell division. The extracellular parasite discharges transmembrane proteins onto its surface to mediate motility and invasion. These are shed by intramembrane cleavage, a process associated with invasion but otherwise poorly understood. Functional analysis of Toxoplasma rhomboid 4, a surface intramembrane protease, by conditional overexpression of a catalytically inactive form produced a profound block in replication. This was completely rescued by expression of the cleaved cytoplasmic tail of Toxoplasma or Plasmodium apical membrane antigen 1 (AMA1). These results reveal an unexpected function for AMA1 in parasite replication and suggest that invasion proteins help to promote parasite switch from an invasive to a replicative mode.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0036-8075eISSN: 1095-9203DOI: 10.1126/science.1199284Titel-ID: cdi_proquest_miscellaneous_907155627
- Format
- –
- Schlagworte
- Antigens, Antigens, Protozoan - chemistry, Antigens, Protozoan - genetics, Antigens, Protozoan - metabolism, Biological and medical sciences, Blocking, Cell Cycle, Cell Division, Cell Membrane - metabolism, Cell membranes, Cells, Cultured, Cleavage, Daughter cells, Fibroblasts - parasitology, Fundamental and applied biological sciences. Psychology, Gene expression, Humans, Life cycle. Host-agent relationship. Pathogenesis, Membrane Proteins - chemistry, Membrane Proteins - genetics, Membrane Proteins - metabolism, Membranes, Movement, Mutant Proteins - metabolism, Organelles, Parasite hosts, Parasites, Parasitic protozoa, Parasitism, Phenotypes, Plasmodium, Plasmodium falciparum, Proteins, Protozoa, Protozoan Proteins - chemistry, Protozoan Proteins - genetics, Protozoan Proteins - metabolism, Replication, Serine Proteases - genetics, Serine Proteases - metabolism, Signal Transduction, Switches, Toxoplasma, Toxoplasma - cytology, Toxoplasma - growth & development, Toxoplasma - physiology, Vacuoles