Details

Autor(en) / Beteiligte

• Tanabe, Atsuhiro
• Shiraishi, Mitsuya
• Negishi, Manabu
• Saito, Naoaki
• Tanabe, Mitsuo
• Sasaki, Yasuharu

Titel

MARCKS dephosphorylation is involved in bradykinin-induced neurite outgrowth in neuroblastoma SH-SY5Y cells

Ist Teil von

• Journal of cellular physiology, 2012-02, Vol.227 (2), p.618-629

Ort / Verlag

Hoboken: Wiley Subscription Services, Inc., A Wiley Company

Erscheinungsjahr

2012

Quelle

MEDLINE

Beschreibungen/Notizen

• Bradykinin (BK) plays a major role in producing peripheral sensitization in response to peripheral inflammation and in pain transmission in the central nerve system (CNS). Because BK activates protein kinase C (PKC) through phospholipase C (PLC)‐β and myristoylated alanine‐rich C kinase substrate (MARCKS) has been found to be a substrate of PKC, we explored the possibility that BK could induce MARCKS phosphorylation and regulate its function. BK stimulation induced transient MARCKS phosphorylation on Ser159 with a peak at 1 min in human neuroblastoma SH‐SY5Y cells. By contrast, PKC activation by the phorbol ester phorbol 12,13‐dibutyrate (PDBu) elicited MARCKS phosphorylation which lasted more than 10 min. Western blotting analyses and glutathione S‐transferase (GST) pull‐down analyses showed that the phosphorylation by BK was the result of activation of the PKC‐dependent RhoA/Rho‐associated coiled‐coil kinase (ROCK) pathway. Protein phosphatase (PP) 2A inhibitors calyculin A and fostriecin inhibited the dephosphorylation of MARCKS after BK‐induced phosphorylation. Moreover, immunoprecipitation analyses showed that PP2A interacts with MARCKS. These results indicated that PP2A is the dominant PP of MARCKS after BK stimulation. We established SH‐SY5Y cell lines expressing wild‐type MARCKS and unphosphorylatable MARCKS, and cell morphology changes after cell stimulation were studied. PDBu induced lamellipodia formation on the neuroblastoma cell line SH‐SY5Y and the morphology was sustained, whereas BK induced neurite outgrowth of the cells via lamellipodia‐like actin accumulation that depended on transient MARCKS phosphorylation. Thus these findings show a novel BK signal cascade—that is, BK promotes neurite outgrowth through transient MARCKS phosphorylation involving the PKC‐dependent RhoA/ROCK pathway and PP2A in a neuroblastoma cell line. J. Cell. Physiol. 227: 618–629, 2012. © 2011 Wiley Periodicals, Inc.