Acta biochimica et biophysica Sinica, 2011-12, Vol.43 (12), p.930-939
2011
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- Autor(en) / Beteiligte
- Titel
- The influence of hyaluronic acid on vascular endothelial cell proliferation and the relationship with ezrin/merlin expression
- Ist Teil von
- Acta biochimica et biophysica Sinica, 2011-12, Vol.43 (12), p.930-939
- Ort / Verlag
- China: Oxford University Press
- Erscheinungsjahr
- 2011
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- It has been established that hyaluronic acid （HA） glycans （nHA） and oligosaccharide （oHA） exert different effects on the biological function of the vascular endo- thelial cell （EC）, resulting in altered regulation of angio- genesis. However, the specific mechanism is still unclear. Our study focused on the effects of nHA and oHA on the ezrin and merlin proteins in EC. The expression of ezrin and merlin was silenced by siRNA, and the regu- lation on EC growth as well as the mRNA expression and activation （phosphorylation） of ezrin and merlin stimulated by oHA and nHA was investigated. The results revealed that when treated with nHA, there was no significant change in ezrin expression or activation. After being treated with oHA, the expression and acti- vation of ezrin were definitively increased whereas there were no obvious changes in merlin expression （including its phosphorylation）. With ezrin expression silenced, the expression of merlin as well as its phosphorylation levels in nHA-stimulated human umbilical vein endo- thelial cells were notably elevated, while there was no significant change induced by oHA. With merlin expression silenced, no obvious change was found in the expression of ezrin （including its phosphorylation） induced by nHA. Conversely, the expression of ezrin and its activation was significantly improved after being treated with oHA. The results suggest that the mechan- ism for the promotion of EC proliferation by oHA is likely related to the expression and activation of ezrin, and the inhibition of EC proliferation by nHA is likely related to the expression and activation of merlin.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1672-9145eISSN: 1745-7270DOI: 10.1093/abbs/gmr094Titel-ID: cdi_proquest_miscellaneous_906151158
- Format
- –
- Schlagworte
- Cell Proliferation - drug effects, Cells, Cultured, Cytoskeletal Proteins - drug effects, Cytoskeletal Proteins - genetics, Cytoskeletal Proteins - metabolism, Endothelium, Vascular - cytology, Endothelium, Vascular - metabolism, Gene Knockdown Techniques, Humans, Hyaluronic Acid - pharmacology, mRNA表达, Neurofibromin 2 - drug effects, Neurofibromin 2 - genetics, Neurofibromin 2 - metabolism, Oligosaccharides - pharmacology, Phosphorylation - drug effects, Polysaccharides - pharmacology, RNA, Messenger - drug effects, RNA, Messenger - metabolism, RNA, Small Interfering - genetics, 内皮细胞增殖, 梅林, 纳米羟基磷灰石, 蛋白, 血管内皮细胞, 血管发生, 透明质酸