Details

Autor(en) / Beteiligte

• Seki, Toshihiko
• Ideta, Ritsuro
• Shibuya, Masabumi
• Adachi, Kenji

Titel

Isolation and Characterization of cDNA for an Androgen-Regulated mRNA in the Flank Organ of Hamsters

Ist Teil von

• Journal of investigative dermatology, 1991-06, Vol.96 (6), p.926-931

Ort / Verlag

Danvers, MA: Elsevier Inc

Erscheinungsjahr

1991

Quelle

MEDLINE

Beschreibungen/Notizen

• Flank organs of hamster are useful for studying androgen-dependent growth of hair follicles and sebaceous glands. To elucidate the mechanism of gene expression regulated by androgen, we constructed a cDNA library from flank organs of male hamsters and screened by a differential hybridization method using cDNA probes from normal and castrated males. We isolated a cDNA clone, termed FAR-17a, whose expression was found to be highly sensitive to androgen. FAR-17a mRNA of 1.8kb was reduced after castration and reappeared after testosterone treatments. Among several tis- sues examined, FAR-17a gene was expressed at a high level in flank organ and a low level in testis and earlobe. FAR-17a probe detected a few fragments in genomic DNA of hamster, mouse, suncus, pig, and human, suggesting that this gene is phylogenetically conserved. The sequence of FAR-17a cDNA predicts a protein of 231 amino acids (27,216 daltons) having basic properties. The deduced protein has no significant homologies to proteins previously described.