Molecular microbiology, 2011-07, Vol.81 (2), p.457-472
2011
Details
- Autor(en) / Beteiligte
- Titel
- The combined actions of the copper‐responsive repressor CsoR and copper‐metallochaperone CopZ modulate CopA‐mediated copper efflux in the intracellular pathogen Listeria monocytogenes
- Ist Teil von
- Molecular microbiology, 2011-07, Vol.81 (2), p.457-472
- Ort / Verlag
- Oxford, UK: Blackwell Publishing Ltd
- Erscheinungsjahr
- 2011
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Summary We have characterized the csoR‐copA‐copZ copper resistance operon of the important human intracellular pathogen Listeria monocytogenes. Transcription of the operon is specifically induced by copper, and mutants lacking the P1‐type ATPase CopA have reduced copper tolerance and over‐accumulate copper relative to wild type. The copper‐responsive repressor CsoR autoregulates transcription by binding to a single 32 bp site spanning the −10 and −35 elements of the promoter. Copper co‐ordination by CsoR derepresses transcription of the operon and alters CsoR:DNA complex assembly as determined by DNase I footprinting and electrophoretic mobility shift assays, with some DNA‐binding capacity being retained in the presence of 2 mole equivalents of copper. Analysis of the CsoR copper sensory site demonstrated that substitution of Cys42 with Ala generated a CsoR variant that was unresponsive to copper. Importantly, in the absence of CopZ, copper responsiveness of csoR‐copA‐copZ expression is substantially increased, implying that CopZ reduces the access of CsoR to copper. Furthermore, CopZ is shown to confer copper resistance in mutants lacking copper‐inducible csoR‐copA‐copZ expression, thus providing protection from the deleterious effects of copper within the cytoplasm.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0950-382XeISSN: 1365-2958DOI: 10.1111/j.1365-2958.2011.07705.xTitel-ID: cdi_proquest_miscellaneous_904482870
- Format
- –
- Schlagworte
- Amino Acid Sequence, Amino Acid Substitution - genetics, Animal Structures - microbiology, Animals, Artificial Gene Fusion, Bacterial Load, Bacterial Proteins - genetics, Bacterial Proteins - metabolism, Bacteriology, beta-Galactosidase - genetics, beta-Galactosidase - metabolism, Biological and medical sciences, Cells, Copper, Copper - metabolism, Disease Models, Animal, DNA Footprinting, DNA, Bacterial - metabolism, Electrophoretic Mobility Shift Assay, Fundamental and applied biological sciences. Psychology, Gene Expression Regulation, Bacterial, Genes, Reporter, Gram-positive bacteria, Listeria monocytogenes, Listeria monocytogenes - genetics, Listeria monocytogenes - metabolism, Listeria monocytogenes - pathogenicity, Listeriosis - microbiology, Listeriosis - mortality, Listeriosis - pathology, Metallochaperones - genetics, Metallochaperones - metabolism, Mice, Microbiology, Miscellaneous, Molecular Sequence Data, Mutagenesis, Site-Directed, Mutant Proteins - genetics, Mutant Proteins - metabolism, Mutation, Operon, Promoter Regions, Genetic, Protein Binding, Repressor Proteins - genetics, Repressor Proteins - metabolism, Rodent Diseases - microbiology, Rodent Diseases - mortality, Rodent Diseases - pathology, Sequence Alignment, Survival Analysis