Details

Autor(en) / Beteiligte

• Carrillo Oesterreich, Fernando
• Preibisch, Stephan
• Neugebauer, Karla M.

Titel

Global Analysis of Nascent RNA Reveals Transcriptional Pausing in Terminal Exons

Ist Teil von

• Molecular cell, 2010-11, Vol.40 (4), p.571-581

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2010

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Pre-mRNA splicing is catalyzed by the spliceosome, which can assemble on pre-mRNA cotranscriptionally. However, whether splicing generally occurs during transcription has not been addressed. Indeed, splicing catalysis is expected to occur posttranscriptionally in yeast, where the shortness of terminal exons should leave insufficient time for splicing. Here, we isolate endogenous S. cerevisiae nascent RNA and determine gene-specific splicing efficiencies and transcription profiles, using high-density tiling microarrays. Surprisingly, we find that splicing occurs cotranscriptionally for the majority of intron-containing genes. Analysis of transcription profiles reveals Pol II pausing within the terminal exons of these genes. Intronless and inefficiently spliced genes lack this pause. In silico simulations of transcription and splicing kinetics confirm that this pausing event provides sufficient time for splicing before termination. The discovery of terminal exon pausing demonstrates functional coupling of transcription and splicing near gene ends. ► Endogenous S. cerevisiae nascent RNA was isolated by chromatin fractionation ► A majority of genes are cotranscriptionally spliced ► RNA Pol II pauses at terminal exons ► In silico simulations validate cotranscriptional splicing