Details

Autor(en) / Beteiligte

• Koshkina, T E
• Baranova, E N
• Usacheva, E A
• Zavriev, S K

Titel

A Point Mutation in the Coat Protein Gene Affects Long-Distance Transport of the Tobacco Mosaic Virus

Ist Teil von

• Molecular biology (New York), 2003-07, Vol.37 (4), p.631-636

Ort / Verlag

New York: Springer Nature B.V

Erscheinungsjahr

2003

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• A mutation resulting in substitution of positively charged Lys53 with negatively charged Glu in the coat protein was introduced in the infectious cDNA copy of the genome of wild-type tobacco mosaic virus strain U1. Kinetic analysis of long-distance virus transport in plants showed that systemic spread of the mutant virus was delayed by 5-6 days as compared with the wild-type one. On evidence of RNA sequencing in the mutant progeny, Glu50 of the coat protein was substituted with Lys after passage I to compensate for the loss of the positive charge at position 53. Electron microscopy revealed atypical inclusions (rodlike structures, multiple electron-dense globular particles) in the nuclear interchromatin space of leaf mesophyll cells infected with the mutant but not with the wild-type virus.[PUBLICATION ABSTRACT]