Details

Autor(en) / Beteiligte

• Tormo, N
• Solano, C
• Benet, I
• Nieto, J
• de la Cámara, R
• López, J
• Garcia-Noblejas, A
• Muñoz-Cobo, B
• Costa, E
• Clari, M A
• Hernández-Boluda, J C
• Remigia, M J
• Navarro, D

Titel

Reconstitution of CMV pp65 and IE-1-specific IFN-γ CD8 and CD4 T-cell responses affording protection from CMV DNAemia following allogeneic hematopoietic SCT

Ist Teil von

• Bone marrow transplantation (Basingstoke), 2011-11, Vol.46 (11), p.1437-1443

Ort / Verlag

London: Nature Publishing Group UK

Erscheinungsjahr

2011

Quelle

MEDLINE

Beschreibungen/Notizen

• Threshold levels of CMV-specific T-cell populations presumably affording protection from active CMV infection in allo-SCT recipients have been proposed, but lack extensive validation. We quantified CMV pp65 and immediate-early 1-specific IFN-γ CD8 + and CD4 + T cell responses at days +30, +60 and +90 after transplantation in 133 patients, and established cutoff cell levels protecting from CMV DNAemia within the first 120 days after transplantation. No patients showing IFN-γ CD8 + or IFN-γ CD4 + T-cell counts >1.0 and >1.2 cells/μL, respectively, developed a subsequent episode of CMV DNAemia. Initial or recurrent episodes of CMV DNAemia occurred in the face of IFN-γ T-cell levels below defined thresholds. Negative predictive values at day +30 for the IFN-γ CD8 + and CD4 + T-cell markers were 68.1 and 61.8%, respectively. Recipients of grafts from CMV seropositive, related or HLA-matched donors, or receiving non-myeloablative conditioning had nonsignificant tendencies to reach more frequently protective levels of both T-cell subsets at early and late (day +365) times after transplantation. The use of anti-thymocyte globulin and umbilical cord blood transplantation were associated with impaired CMV-specific T-cell reconstitution. CMV-specific IFN-γ CD8 + and CD4 + T-cell recovery occurred irrespective of detectable CMV DNAemia.