Details

Autor(en) / Beteiligte

• Bussotti, Filippo
• Pollastrini, Martina
• Cascio, Chiara
• Desotgiu, Rosanna
• Gerosa, Giacomo
• Marzuoli, Riccardo
• Nali, Cristina
• Lorenzini, Giacomo
• Pellegrini, Elisa
• Carucci, Maria Giovanna
• Salvatori, Elisabetta
• Fusaro, Lina
• Piccotto, Massimo
• Malaspina, Paola
• Manfredi, Alice
• Roccotello, Enrica
• Toscano, Stefania
• Gottardini, Elena
• Cristofori, Antonella
• Fini, Alessio
• Weber, Daniel
• Baldassarre, Valentina
• Barbanti, Lorenzo
• Monti, Andrea
• Strasser, Reto J.

Titel

Conclusive remarks. Reliability and comparability of chlorophyll fluorescence data from several field teams

Ist Teil von

• Environmental and experimental botany, 2011-11, Vol.73, p.116-119

Ort / Verlag

Amsterdam: Elsevier B.V

Erscheinungsjahr

2011

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• ▶ Different surveyors using different instruments for chlorophyll fluorescence analysis may have results not comparable. ▶ The parameters FV/FM is the more consistent across different instruments and operators. ▶ The intercalibration of the instruments and the adoption of proper field protocols are prerequisite indispensable before a large survey. Two field exercises were carried out to compare chlorophyll a fluorescence measurements taken in the field by field teams working on the same project. In the first exercise (2007, Passo Pura, Ampezzo, Udine, Northern Italy) the operators took measurements on the same leaf areas (maintaining fixed leaf clips); in the second (2009, Monterotondo Marittimo, Grosseto, Central Italy) the teams worked independently, but addressing a common research question. The results of the first exercise showed that: (a) F V/ F M was stable and had little variation among teams and instruments; (b) the results from the different teams correlated well; (c) the most suitable parameters of fast kinetics analysis are those measured on the normalized fluorescence transients. In the second exercise, when the teams worked independently, the results were much more variable and the correlations between measurements of different operators were weak. These results suggest that field chlorophyll a fluorescence measurements taken by different teams/operators can be comparable only if particular care is taken to the internal variability of the samples and a standardized sampling strategy is applied. A statistically sound representation of a population can be then reached.