Details

Autor(en) / Beteiligte

• Jeong, Mi Jin
• Song, Hyun Jin
• Park, Dong Jin
• Min, Ji Yun
• Jo, Jin Seong
• Kim, Bo Min
• Kim, Hak Gon
• Kim, Yong Duck
• Kim, Ru Mi
• Karigar, Chandrakant S
• Choi, Myung Suk

Titel

High frequency plant regeneration following abnormal shoot organogenesis in the medicinal tree Hovenia dulcis

Ist Teil von

• Plant cell, tissue and organ culture, 2009-07, Vol.98 (1), p.59-65

Ort / Verlag

Dordrecht: Dordrecht : Springer Netherlands

Erscheinungsjahr

2009

Quelle

springer (창간호~2014)

Beschreibungen/Notizen

• An efficient plant regeneration protocol for shoot organogenesis from Hovenia dulcis callus cultures was established. Induction of organogenic callus was achieved on Murashige and Skoog (MS) medium supplemented with 4.65 μM kinetin and 4.5 μM 2,4-dichlorophenoxyacetic acid (2,4-D). Further differentiation of organogenic callus into primordia, shoot-like structures, and plantlets was achieved on MS medium supplemented with 0.23 μM gibberellic acid (GA₃) and 0.46 μM kinetin. Numerous abnormal shoots developed upon transfer of callus to MS medium containing cytokinins, and these failed to grow further into whole plantlets. However, transfer of 'abnormal' shoots to a fresh MS medium lacking cytokinins resulted in growth of normal shoots. Elongated shoots subsequently were rooted in basal MS medium, and whole plantlets were established in a soil mix. Analysis of regenerated plants using random amplified polymorphic DNA (RAPD) confirmed the genetic stability of these regenerant plantlets.