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Amplification Strategy Using Aggregates of Ferrocene-Containing Cationic Polythiophene for Sensitive and Specific Electrochemical Detection of DNA
Ist Teil von
Analytical chemistry (Washington), 2011-11, Vol.83 (21), p.8086-8092
Ort / Verlag
Washington, DC: American Chemical Society
Erscheinungsjahr
2011
Link zum Volltext
Quelle
MEDLINE
Beschreibungen/Notizen
We report a new electrochemical amplification strategy for an ultrasensitive electrochemical detection of DNA sequences using aggregates composed of a water-soluble, ferrocene-functionalized polythiophene. A two-step hybridization is performed at one addressing surface with PNA capture probes whereas the electrochemical detection is done on an electrode nearby. Specific and quantitative detection of DNA targets with a detection limit of 4 × 10–16 M (about 4 zeptomoles or about 2500 copies of oligonucleotides) was achieved.