Proteomics (Weinheim), 2011-11, Vol.11 (21), p.4139-4154
2011
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- Autor(en) / Beteiligte
- Titel
- Proteomic analysis of oligodendrogliomas expressing a mutant isocitrate dehydrogenase-1
- Ist Teil von
- Proteomics (Weinheim), 2011-11, Vol.11 (21), p.4139-4154
- Ort / Verlag
- Weinheim: WILEY-VCH Verlag
- Erscheinungsjahr
- 2011
- Quelle
- Wiley-Blackwell Journals
- Beschreibungen/Notizen
- Gliomas are primary tumors of the human central nervous system with unknown mechanisms of progression. Isocitrate dehydrogenase‐1 (IDH1) mutation is frequent in diffuse gliomas such as oligodendrogliomas. To gain insights into the physiopathology of oligodendrogliomas that have a better prognosis than other diffuse gliomas, we combined microdissection, 2‐D DIGE and MS/MS focusing on proteome alterations associated with IDH1 mutation. We first compared tumor tissues (TT) and minimally infiltrated parenchymal tissues (MIT) of four IDH1‐mutated oligodendrogliomas to verify whether proteins specific to oligodendroglioma tumor cells could be identified from one patient to another. This study resulted in identification of 68 differentially expressed proteins, with functions related to growth of tumor cells in a nervous parenchyma. We then looked for proteins distinctly expressed in TT harboring either mutant (oligodendrogliomas, n=4) or wild‐type IDH1 (oligodendroglial component of malignant glio‐neuronal tumors, n=4). This second analysis resulted in identification of distinct proteome patterns composed of 42 proteins. Oligodendrogliomas with a mutant IDH1 had noteworthy enhanced expression of enzymes controlling aerobic glycolysis and detoxification, and anti‐apoptosis proteins. In addition, the mutant IDH1 migrated differently from the wild‐type IDH1 form. Comparative proteomic analysis might thus be suitable to identify proteome alterations associated with a well‐defined mutation.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1615-9853, 1862-8346eISSN: 1615-9861DOI: 10.1002/pmic.201000646Titel-ID: cdi_proquest_miscellaneous_900626661
- Format
- –
- Schlagworte
- 2-D DIGE, Adult, Aged, Aged, 80 and over, Analytical, structural and metabolic biochemistry, Biological and medical sciences, Brain cancer, Brain Neoplasms - enzymology, Brain Neoplasms - genetics, Brain Neoplasms - metabolism, Brain Neoplasms - pathology, Brain tumors, Cell biology, Central nervous system, Dehydrogenases, Detoxification, Enzymes, Female, Fundamental and applied biological sciences. Psychology, Gene Expression Regulation, Neoplastic, Glioma, Gliomas, Glycolysis, Human, Humans, IDH1, Isocitrate Dehydrogenase - genetics, Male, Medical sciences, Metabolic Networks and Pathways, Middle Aged, Miscellaneous, Mutation, Neurology, oligodendroglioma, Oligodendroglioma - enzymology, Oligodendroglioma - genetics, Oligodendroglioma - metabolism, Oligodendroglioma - pathology, Parenchyma, Prognosis, Proteins, Proteome - genetics, Proteome - metabolism, proteomics, Proteomics - methods, Tandem Mass Spectrometry - methods, Therapeutic applications, Tumor cells, Tumors, Tumors of the nervous system. Phacomatoses, Two-Dimensional Difference Gel Electrophoresis - methods