Details

Autor(en) / Beteiligte

• Cao, Hong
• Liu, Daling
• Mo, Xuemei
• Xie, Chunfang
• Yao, Dongsheng

Titel

fungal enzyme with the ability of aflatoxin B₁ conversion: Purification and ESI-MS/MS identification

Ist Teil von

• Microbiological research, 2011-09, Vol.166 (6), p.475-483

Ort / Verlag

Germany: Elsevier GmbH

Erscheinungsjahr

2011

Quelle

MEDLINE

Beschreibungen/Notizen

• Armillariella tabescens, a Chinese edible and medicinal fungus, whose multienzyme exist ability of AFB₁-converting, and ADTZ (aflatoxin-detoxizyme) had previously purified from the A. tabescens multienzyme monitored by AFB₁ conversion. However, the enzyme now confirmed an oxidase and renamed aflatoxin-oxidase (AFO). In this paper, AFO was purified by an economical and practical three-step procedure monitored by AFB₁ conversion. And ESI-MS/MS analysis was done for identification of AFO. The following database searching (Protein Blast on NCBI) results did not show any homologous oxidase protein, which implied that AFO was mostly a new oxidase differing from other reported aflatoxin-converting enzymes such as fungal laccase and horse radish peroxidase. HPTLC analysis of the purified AFO activity suggested that the enzyme reacted at the bisfuran ring of AFB₁ which was the key toxic structure. Therefore, all these investigations implied a new choice for biodegradation of aflatoxin in foods and feeds with the practical application of AFO.