Details

Autor(en) / Beteiligte

• Landeta, Gerardo
• Reverón, Inés
• Carrascosa, Alfonso V.
• Rivas, Blanca de las
• Muñoz, Rosario

Titel

Use of recA gene sequence analysis for the identification of Staphylococcus equorum strains predominant on dry-cured hams

Ist Teil von

• Food microbiology, 2011-09, Vol.28 (6), p.1205-1210

Ort / Verlag

Kidlington: Elsevier Ltd

Erscheinungsjahr

2011

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Spanish dry-cured ham is an uncooked meat product highly appreciated due to its characteristics flavour. In this study, we examined the accuracy of biochemical tests and 16S rDNA sequencing in the identification of 56 staphylococcal strains isolated during industrial Spanish dry-cured ham processes. Important differences were observed comparing genotypic and phenotypic data. Staphylococcus xylosus was the prevalent species identified by biochemical methods (87.5%), however, sequencing of the 16S rDNA resulted in an unambiguous identification of Staphylococcus equorum (73.2%) and Staphylococcus vitulinus (8.9%) strains. Reliable identification of meat staphylococci, mainly among S. xylosus and S. equorum strains could be also achieved by means of recA gene sequence comparison. Two degenerate primers previously described for lactic acid bacteria were used to amplify an internal fragment of the recA gene. This fragment was amplified from twelve staphylococcal type strains representing frequent meat species. The results indicated that recA sequencing is an adequate method to discriminate among meat staphylococci. In addition, S. xylosus and S. equorum strains could be more accurately discriminated by recA sequencing than 16S rDNA or sodA sequencing. The S. equorum sequence diversity showed at the intra-species level by recA gene sequencing confirmed the high heterogeneity described among S. equorum strains. ► Identification differences were observed comparing phenotypic and genotypic data. ► Staphylococcus equorum was the prevalent species identified by molecular methods. ► Reliable identification could be achieved by recA gene sequence comparison. ► Staphylococcus xylosus and S. equorum strains are accurately discriminated by recA sequencing.