The Plant journal : for cell and molecular biology, 2011-10, Vol.68 (2), p.302-313
2011
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Details
- Autor(en) / Beteiligte
- Titel
- Soybean NAC transcription factors promote abiotic stress tolerance and lateral root formation in transgenic plants
- Ist Teil von
- The Plant journal : for cell and molecular biology, 2011-10, Vol.68 (2), p.302-313
- Ort / Verlag
- Oxford, UK: Blackwell Publishing Ltd
- Erscheinungsjahr
- 2011
- Quelle
- Wiley Online Library Journals Frontfile Complete
- Beschreibungen/Notizen
- Summary NAC transcription factors play important roles in plant growth, development and stress responses. Previously, we identified multiple NAC genes in soybean (Glycine max). Here, we identify the roles of two genes, GmNAC11 and GmNAC20, in stress responses and other processes. The two genes were differentially induced by multiple abiotic stresses and plant hormones, and their transcripts were abundant in roots and cotyledons. Both genes encoded proteins that localized to the nucleus and bound to the core DNA sequence CGT[G/A]. In the protoplast assay system, GmNAC11 acts as a transcriptional activator, whereas GmNAC20 functions as a mild repressor; however, the C‐terminal end of GmANC20 has transcriptional activation activity. Over‐expression of GmNAC20 enhances salt and freezing tolerance in transgenic Arabidopsis plants; however, GmNAC11 over‐expression only improves salt tolerance. Over‐expression of GmNAC20 also promotes lateral root formation. GmNAC20 may regulate stress tolerance through activation of the DREB/CBF–COR pathway, and may control lateral root development by altering auxin signaling‐related genes. GmNAC11 probably regulates DREB1A and other stress‐related genes. The roles of the two GmNAC genes in stress tolerance were further analyzed in soybean transgenic hairy roots. These results provide a basis for genetic manipulation to improve the agronomic traits of important crops.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0960-7412eISSN: 1365-313XDOI: 10.1111/j.1365-313X.2011.04687.xTitel-ID: cdi_proquest_miscellaneous_898202851
- Format
- –
- Schlagworte
- Agronomy, Arabidopsis - genetics, Arabidopsis - metabolism, Biological and medical sciences, Cell Nucleus - metabolism, Cotyledon - genetics, Cotyledon - growth & development, Cotyledon - metabolism, Crop science, DNA-Binding Proteins - genetics, DNA-Binding Proteins - metabolism, Droughts, Flowers - genetics, Flowers - growth & development, Flowers - metabolism, Freezing, Fundamental and applied biological sciences. Psychology, Gene expression, Gene Expression Regulation, Plant - genetics, Glycine max - genetics, Glycine max - growth & development, Glycine max - metabolism, Green Fluorescent Proteins, Indoleacetic Acids - metabolism, lateral root, NAC protein, Nucleotide Motifs - genetics, Plant growth, Plant Growth Regulators - metabolism, Plant physiology and development, Plant Proteins - genetics, Plant Proteins - metabolism, Plant Roots - genetics, Plant Roots - growth & development, Plant Roots - metabolism, Plant Stems - genetics, Plant Stems - growth & development, Plant Stems - metabolism, Plants, Genetically Modified, Protoplasts, Salt Tolerance, Seedlings - genetics, Seedlings - growth & development, Seedlings - metabolism, Sodium Chloride - pharmacology, soybean, Soybean Proteins - genetics, Soybean Proteins - metabolism, Soybeans, stress tolerance, Stress, Physiological - physiology, transcription factor, Transcription Factors - genetics, Transcription Factors - metabolism, Transcriptional Activation, Transgenic plants