Molecular and cellular endocrinology, 2010-04, Vol.317 (1), p.1-7
2010
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- T3 acutely increases GH mRNA translation rate and GH secretion in hypothyroid rats
- Ist Teil von
- Molecular and cellular endocrinology, 2010-04, Vol.317 (1), p.1-7
- Ort / Verlag
- Ireland: Elsevier Ireland Ltd
- Erscheinungsjahr
- 2010
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Cytoskeleton controls the stability of transcripts, by mechanisms that involve mRNAs and eEF1A attachment to it. Besides, it plays a key role in protein synthesis and secretion, which seems to be impaired in somatotrophs of hypothyroid rats, whose cytoskeleton is disarranged. This study investigated the: eEF1A and GH mRNA binding to cytoskeleton plus GH mRNA translation rate and GH secretion, in sham-operated and thyroidectomized rats treated with T3 or saline, and killed 30 min thereafter. Thyroidectomy reduced: (a) pituitary F-actin content, and eEF1A plus GH mRNA binding to it; (b) GH mRNA recruitment to polysome; and (c) liver IGF-I mRNA expression, indicating that GH mRNA stability and translation rate, as well as GH secretion were impaired. T3 acutely reversed all these changes, which points toward a nongenomic action of T3 on cytoskeleton rearrangement, which might contribute to the increase on GH mRNA translation rate and GH secretion.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0303-7207eISSN: 1872-8057DOI: 10.1016/j.mce.2009.12.005Titel-ID: cdi_proquest_miscellaneous_896186179
- Format
- –
- Schlagworte
- Actins - metabolism, Animals, Body Weight - drug effects, Cytoskeleton, Cytoskeleton - drug effects, Cytoskeleton - metabolism, eEF1A, Gene Expression Regulation - drug effects, Growth Hormone - genetics, Growth Hormone - secretion, Hypothyroidism - genetics, Hypothyroidism - pathology, Insulin-Like Growth Factor I - genetics, Insulin-Like Growth Factor I - metabolism, Liver - drug effects, Liver - metabolism, Liver - pathology, Male, Nongenomic action, Organ Size - drug effects, Peptide Elongation Factor 1 - metabolism, Pituitary Gland - drug effects, Pituitary Gland - metabolism, Pituitary Gland - pathology, Polyribosomes - drug effects, Polyribosomes - metabolism, Polysomes, Protein Binding - drug effects, Protein Biosynthesis - drug effects, Rats, Rats, Wistar, RNA, Messenger - genetics, RNA, Messenger - metabolism, Triiodothyronine - pharmacology