Nature biotechnology, 2011-08, Vol.29 (8), p.750-756
2011
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Cell-surface markers for the isolation of pancreatic cell types derived from human embryonic stem cells
- Ist Teil von
- Nature biotechnology, 2011-08, Vol.29 (8), p.750-756
- Ort / Verlag
- New York, NY: Nature Publishing Group
- Erscheinungsjahr
- 2011
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Using a flow cytometry-based screen of commercial antibodies, we have identified cell-surface markers for the separation of pancreatic cell types derived from human embryonic stem (hES) cells. We show enrichment of pancreatic endoderm cells using CD142 and of endocrine cells using CD200 and CD318. After transplantation into mice, enriched pancreatic endoderm cells give rise to all the pancreatic lineages, including functional insulin-producing cells, demonstrating that they are pancreatic progenitors. In contrast, implanted, enriched polyhormonal endocrine cells principally give rise to glucagon cells. These antibodies will aid investigations that use pancreatic cells generated from pluripotent stem cells to study diabetes and pancreas biology.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1087-0156eISSN: 1546-1696DOI: 10.1038/nbt.1931Titel-ID: cdi_proquest_miscellaneous_893287375
- Format
- –
- Schlagworte
- Animals, Antibodies - metabolism, Antigens, CD - metabolism, Biological and medical sciences, Biological markers, Biomarkers - metabolism, Biotechnology, Cell Separation - methods, Cells, Cultured, Cellular biology, Embryonic stem cells, Embryonic Stem Cells - cytology, Embryonic Stem Cells - metabolism, Embryos, Endoderm - cytology, Flow Cytometry, Fundamental and applied biological sciences. Psychology, Humans, Mice, Mice, SCID, Microscopy, Fluorescence, Pancreas, Pancreas - cytology, Physiological aspects, Stem cells, Transplantation, Heterologous