Details

Autor(en) / Beteiligte

• DE WIT, Harry
• NABBE, Karin C. A. M
• KOOREN, Jurgen A
• ADRIAANSEN, HenkJ
• ROELANDSE-KOOP, Elianne A
• SCHUITEMAKER, Joost H. N
• HOFFMANN, Johannes J. M. L

Titel

Reference Values of Fetal Erythrocytes in Maternal Blood During Pregnancy Established Using Flow Cytometry

Ist Teil von

• American journal of clinical pathology, 2011-10, Vol.136 (4), p.631-636

Ort / Verlag

Chicago, IL: American Society of Clinical Pathologists

Erscheinungsjahr

2011

Quelle

Oxford Journals 2020 Medicine

Beschreibungen/Notizen

• The aim of our study was to assess the fetal RBC count in maternal blood during uncomplicated pregnancies from 26 weeks onward. We used a flow cytometric method specifically designed for use in a routine hematology analyzer. Pregnant women were recruited through midwives. The participating laboratories used the FMH QuikQuant method (Trillium Diagnostics, Brewer, ME) in a CELL-DYN Sapphire hematology analyzer (Abbott Diagnostics, Santa Clara, CA). The method is based on a monoclonal antibody to hemoglobin F. Flow cytometric data were analyzed by 2 independent observers. The 95th percentile reference range was estimated according to Clinical and Laboratory Standards Institute guidelines. A total of 236 samples were statistically analyzed. Gestational ages ranged from 21.6 to 41 weeks (mean, 32.0 weeks), and the fetal RBC count in maternal blood ranged from 0.00% to 0.50% (median, 0.025%). The fetal RBC count in maternal blood shows no correlation with gestational age. The established reference range during normal pregnancy is less than 0.125%.