Details

Autor(en) / Beteiligte

• Magnani, Natalia D.
• Marchini, Timoteo
• Tasat, Deborah R.
• Alvarez, Silvia
• Evelson, Pablo A.

Titel

Lung oxidative metabolism after exposure to ambient particles

Ist Teil von

• Biochemical and biophysical research communications, 2011-09, Vol.412 (4), p.667-672

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2011

Quelle

MEDLINE

Beschreibungen/Notizen

• ► Exposure to ambient particles increased the oxidative metabolism in mice lung. ► Increased O2 consumption precedes increased NO production in this model. ► It was accompanied by an increased NADPH oxidase activity and mitochondrial O2 uptake. ► Phospholipid oxidation precedes oxidative damage to proteins in this model. ► O2- production and mitochondrial O2 uptake positively correlates to oxidative damage. The aim of this work was to study the time course of the oxidative metabolism in mice lung after exposure to ambient particles (ROFA). Swiss mice were intranasally instilled with a ROFA suspension (0.20mg/kg). Animals were sacrificed 1 or 3h after the exposure. Eighty percentage of increased oxygen consumption was observed in tissue cubes after 1h of exposure. This observation was accompanied by an increased NADPH oxidase activity (40%) and mitochondrial oxygen consumption in state 3 (19%). NO production by lung homogenates was found to be increased by 43% after 3h of exposure. Phospholipid oxidation in lung homogenates showed a 29% increase after 1h of exposure, while a 30% increase in the carbonyl content was found only after 3h of exposure. Our data show the relative importance of different sources of reactive oxygen species (NADPH oxidase activity and mitochondrial respiration) to the increased tissue oxygen consumption, oxidative damage and antioxidant status observed in an acute model of ROFA particles exposure.