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Reliable and Efficient Procedures for the Conjugation of Biomolecules through Huisgen Azide-Alkyne Cycloadditions
Ist Teil von
Angewandte Chemie International Edition, 2011-09, Vol.50 (38), p.8794-8804
Auflage
International ed. in English
Ort / Verlag
Weinheim: WILEY-VCH Verlag
Erscheinungsjahr
2011
Quelle
MEDLINE
Beschreibungen/Notizen
The CuI‐catalyzed azide–alkyne cycloaddition (CuAAC) has been established as a powerful coupling technology for the conjugation of proteins, nucleic acids, and polysaccharides. Nevertheless, several shortcomings related to the presence of Cu, mainly oxidative degradation by reactive oxygen species and sample contamination by Cu, have been pointed out. This Minireview discusses key aspects found in the development of the efficient and benign functionalization of biomacromolecules through CuAAC, as well as the Cu‐free strain‐promoted azide–alkyne cycloaddition (SPAAC).
Efficient and benign bioconjugation: CuI‐catalyzed azide–alkyne cycloaddition (CuAAC) is an established coupling method because of its high reliability and straightforward experimental procedure. However, the Cu catalyst has often proved detrimental to proteins, nucleic acids, and polysaccharides. CuI ligands and Cu‐free strain‐promoted procedures (SPAAC) have been developed that are efficient and avoid degradation of the resulting bioconjugates.