Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie, 2011-09, Vol.63 (6), p.519-525
2011
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Details
- Autor(en) / Beteiligte
- Titel
- Protective effects of asiaticoside on septic lung injury in mice
- Ist Teil von
- Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie, 2011-09, Vol.63 (6), p.519-525
- Ort / Verlag
- Munich: Elsevier GmbH
- Erscheinungsjahr
- 2011
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Asiaticoside (AS), a major triterpenoid saponin component isolated from Centella asiatica, has been described to exhibit antioxidant and anti-inflammatory activities. The present study aimed to determine the protective effects and the underlying mechanisms of AS on septic lung injury induced by cecal ligation and puncture (CLP). Mice were pretreated with the AS (45 mg/kg) or AS as well as GW9662 at 1 h before CLP, the survival, lung injury, inflammatory mediators and signaling molecules, and Peroxisome proliferator-activated receptor-γ (PPAR-γ) were determined 24 h after CLP. The results showed that AS significantly decreased CLP-induced the mortality, lung pathological damage, the infiltration of mononuclear, polymorphonuclear (PMN) leucocytes and total proteins. Moreover, AS inhibited CLP-induced the activation of mitogen-activated protein kinases (MAPKs) and nuclear factor-κB (NF-κB), the expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) protein in lung tissues, and the production of serum tumor necrosis factor (TNF-α) and interleukin-6 (IL-6). Interestingly, the expression of PPAR-γ protein in lung tissue was up-regulated by AS. Furthermore, GW9662 (the inhibitor of PPAR-γ) significantly reversed these beneficial effects of AS in septic mice. These findings suggest that AS could effectively protect from septic lung injury induced by CLP and the underlying mechanisms might be related to up-regulation of PPAR-γ expression to some extent, which inhibits MAPKs and NF-κB pathway.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0940-2993eISSN: 1618-1433DOI: 10.1016/j.etp.2010.04.002Titel-ID: cdi_proquest_miscellaneous_888118210
- Format
- –
- Schlagworte
- Animals, anti-inflammatory activity, Anti-Inflammatory Agents, Non-Steroidal - administration & dosage, Anti-Inflammatory Agents, Non-Steroidal - isolation & purification, Anti-Inflammatory Agents, Non-Steroidal - therapeutic use, antioxidants, Antioxidants - administration & dosage, Antioxidants - isolation & purification, Antioxidants - therapeutic use, Asiaticoside, Biological and medical sciences, blood serum, Blotting, Western, Bronchoalveolar Lavage Fluid - chemistry, Bronchoalveolar Lavage Fluid - cytology, Cecal ligation and puncture (CLP), Centella - chemistry, Centella asiatica, Cyclooxygenase 2 - biosynthesis, Cytokines - blood, Disease Models, Animal, inducible nitric oxide synthase, interleukin-6, Investigative techniques, diagnostic techniques (general aspects), Leukocyte Count, leukocytes, Lung - drug effects, Lung - enzymology, Lung - immunology, Lung - pathology, Lung Injury - drug therapy, Lung Injury - enzymology, Lung Injury - immunology, Lung Injury - pathology, Male, Medical sciences, Mice, Mice, Inbred Strains, mitogen-activated protein kinase, Molecular Structure, mortality, necrosis, Nitric Oxide Synthase Type II - biosynthesis, Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques, Peroxisome proliferator-activated receptor-γ (PPAR-γ), Pneumonia, Bacterial - drug therapy, Pneumonia, Bacterial - enzymology, Pneumonia, Bacterial - immunology, Pneumonia, Bacterial - pathology, PPAR gamma - biosynthesis, prostaglandin synthase, protective effect, proteins, Septic lung injury, tissues, transcription factor NF-kappa B, Triterpenes - administration & dosage, Triterpenes - isolation & purification, Triterpenes - therapeutic use, tumor necrosis factor-alpha