Bioresource technology, 2011-09, Vol.102 (18), p.8445-8456
2011
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Details
- Autor(en) / Beteiligte
- Titel
- Application of molecular techniques on heterotrophic hydrogen production research
- Ist Teil von
- Bioresource technology, 2011-09, Vol.102 (18), p.8445-8456
- Ort / Verlag
- England: Elsevier Ltd
- Erscheinungsjahr
- 2011
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- This paper reviews the application of molecular techniques in heterotrophic hydrogen production studies. Commonly used molecular techniques are introduced briefly first, including cloning-sequencing after polymerase chain reaction (PCR), denaturing gradient gel electrophoresis (DGGE), terminal-restriction fragment length polymorphism (T-RFLP), fluorescence in situ hybridization (FISH) and quantitative real-time PCR. Application of the molecular techniques in heterotrophic hydrogen production studies are discussed in details, focusing on identification of new isolates for hydrogen production, characterization of microbial compositions in bioreactors, monitoring microbial diversity variation, visualization of microbial distribution in hydrogen-producing granular sludge, and quantification of various microbial populations. Some significant findings in recent hydrogen production studies with the application of molecular techniques are discussed, followed by a research outlook of the heterotrophic biohydrogen field.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0960-8524eISSN: 1873-2976DOI: 10.1016/j.biortech.2011.02.072Titel-ID: cdi_proquest_miscellaneous_888116057
- Format
- –
- Schlagworte
- 16S rDNA, biohydrogen, bioreactors, Biotechnology - methods, denaturing gradient gel electrophoresis, Fe-hydrogenase gene, Fish, Fluorescence, fluorescence in situ hybridization, Focusing, Heterotrophic Processes - physiology, Hydrogen - metabolism, Hydrogen production, Microorganisms, Molecular Biology - methods, Molecular techniques, Monitoring, PCR, polymerase chain reaction, Polymorphism, Research Design, Sludge