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An improved Flp deleter mouse in C57Bl/6 based on Flpo recombinase
Genesis (New York, N.Y. : 2000), 2010-08, Vol.48 (8), p.512-520
Kranz, Andrea
Fu, Jun
Duerschke, Kristin
Weidlich, Stefanie
Naumann, Ronald
Stewart, A. Francis
Anastassiadis, Konstantinos
2010
Details
Autor(en) / Beteiligte
Kranz, Andrea
Fu, Jun
Duerschke, Kristin
Weidlich, Stefanie
Naumann, Ronald
Stewart, A. Francis
Anastassiadis, Konstantinos
Titel
An improved Flp deleter mouse in C57Bl/6 based on Flpo recombinase
Ist Teil von
Genesis (New York, N.Y. : 2000), 2010-08, Vol.48 (8), p.512-520
Ort / Verlag
Hoboken: Wiley Subscription Services, Inc., A Wiley Company
Erscheinungsjahr
2010
Link zum Volltext
Quelle
MEDLINE
Beschreibungen/Notizen
Recently, a codon improved version of the Flpe site specific recombinase, termed Flpo, was reported as having greatly improved performance in mammalian cell applications. However, the degree of improvement could not be estimated because essentially no Flpe activity was observed. Here, we compare Flpe and Flpo accurately in a mammalian cell assay to estimate that Flpo is about five times more active than Flpe and similar to Cre and Dre. Consequently, we generated a Flpo deleter mouse line from the JM8 C57Bl/6 ES cells used in the EUCOMM and KOMP systematic knock‐out programs. In breeding experiments, we show that the Flpo deleter delivers complete recombination using alleles that are incompletely recombined by a commonly used Flpe deleter. This indicates that the Flpo deleter is more efficient. genesis 48:512–520, 2010. © 2010 Wiley‐Liss, Inc.
Sprache
Englisch
Identifikatoren
ISSN: 1526-954X, 1526-968X
eISSN: 1526-968X
DOI: 10.1002/dvg.20641
Titel-ID: cdi_proquest_miscellaneous_888099604
Format
–
Schlagworte
allele design
,
Animals
,
Base Sequence
,
Binding Sites - genetics
,
Cell Line
,
Cells, Cultured
,
conditional mutagenesis
,
DNA Nucleotidyltransferases - genetics
,
DNA Nucleotidyltransferases - metabolism
,
Embryo, Mammalian - cytology
,
Embryo, Mammalian - metabolism
,
Embryonic Stem Cells - cytology
,
Embryonic Stem Cells - metabolism
,
Female
,
Fibroblasts - cytology
,
Fibroblasts - metabolism
,
Gene Expression Regulation, Developmental
,
Gene Knockout Techniques
,
genome engineering
,
Green Fluorescent Proteins - genetics
,
Green Fluorescent Proteins - metabolism
,
Isoenzymes - genetics
,
Isoenzymes - metabolism
,
Male
,
Mice
,
Mice, Inbred C57BL
,
Mice, Transgenic
,
Plasmids - genetics
,
Recombination, Genetic
,
site specific recombination
,
Transfection
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