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Occurrence of a Silicatein Gene in Glass Sponges (Hexactinellida: Porifera)
Ist Teil von
Marine biotechnology (New York, N.Y.), 2011-08, Vol.13 (4), p.810-819
Ort / Verlag
New York: Springer-Verlag
Erscheinungsjahr
2011
Link zum Volltext
Quelle
MEDLINE
Beschreibungen/Notizen
Silicatein genes are involved in spicule formation in demosponges (Demospongiae: Porifera). However, numerous attempts to isolate silicatein genes from glass sponges (Hexactinellida: Porifera) resulted in a limited success. In the present investigation, we performed analysis of potential silicatein/cathepsin transcripts in three different species of glass sponges (
Pheronema raphanus
,
Aulosaccus schulzei
, and
Bathydorus levis
). In total, 472 clones of such transcripts have been analyzed. Most of them represent cathepsin transcripts and only three clones have been found to represent transcripts, which can be related to silicateins. Silicatein transcripts were identified in
A. schulzei
(Hexactinellida; Lyssacinosida; Rosselidae), and the corresponding gene was called
AuSil-Hexa
. Expression of
AuSil-Hexa
in
A. schulzei
was confirmed by real-time PCR. Comparative sequence analysis indicates high sequence identity of the
A. schulzei
silicatein with demosponge silicateins described previously. A phylogenetic analysis indicates that the AuSil-Hexa protein belongs to silicateins. However, the AuSil-Hexa protein contains a catalytic cysteine instead of the conventional serine.