Journal of biomedical materials research. Part A, 2010-04, Vol.93A (1), p.164-174
2010
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- Autor(en) / Beteiligte
- Titel
- Electrical regulation of Schwann cells using conductive polypyrrole/chitosan polymers
- Ist Teil von
- Journal of biomedical materials research. Part A, 2010-04, Vol.93A (1), p.164-174
- Ort / Verlag
- Hoboken: Wiley Subscription Services, Inc., A Wiley Company
- Erscheinungsjahr
- 2010
- Quelle
- Wiley Online Library Journals Frontfile Complete
- Beschreibungen/Notizen
- Electrical stimulation (ES) can dramatically enhance neurite outgrowth through conductive polymers and accelerate peripheral nerve regeneration in animal models of nerve injury. Therefore, conductive tissue engineering graft in combination with ES is a potential treatment for neural injuries. Conductive tissue engineering graft can be obtained by seeding Schwann cells on conductive scaffold. However, when ES is applied through the conductive scaffold, the impact of ES on Schwann cells has never been investigated. In this study, a biodegradable conductive composite made of conductive polypyrrole (PPy, 2.5%) and biodegradable chitosan (97.5%) was prepared in order to electrically stimulate Schwann cells. The tolerance of Schwann cells to ES was examined by a cell apoptosis assay. The growth of the cells was characterized using DAPI staining and a MTT assay. mRNA and protein levels of nerve growth factor (NGF) and brain‐derived neurotrophic factor (BDNF) in Schwann cells were assayed by RT‐PCR and Western blotting, and the amount of NGF and BDNF secreted was determined by an ELISA assay. The results showed that the PPy/chitosan membranes supported cell adhesion, spreading, and proliferation with or without ES. Interestingly, ES applied through the PPy/chitosan composite dramatically enhanced the expression and secretion of NGF and BDNF when compared with control cells without ES. These findings highlight for the first time the possibility of enhancing nerve regeneration in conductive scaffolds through ES‐increased neurotrophin secretion. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res 2010
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1549-3296, 1552-4965eISSN: 1552-4965DOI: 10.1002/jbm.a.32511Titel-ID: cdi_proquest_miscellaneous_883022659
- Format
- –
- Schlagworte
- Animals, BDNF, Biological and medical sciences, Biotechnology, Brain-Derived Neurotrophic Factor - genetics, Brain-Derived Neurotrophic Factor - metabolism, Cell Adhesion - drug effects, Cell Proliferation - drug effects, Cells, Cultured, chitosan, Chitosan - pharmacology, Cranial nerves. Peripheral nerves. Autonomic nervous system, Electric Conductivity, Electric Stimulation, electrical stimulation, Fundamental and applied biological sciences. Psychology, Gene Expression Regulation - drug effects, Health. Pharmaceutical industry, Industrial applications and implications. Economical aspects, Medical sciences, Membranes, Artificial, Microscopy, Electron, Scanning, Miscellaneous, Nerve Growth Factor - genetics, Nerve Growth Factor - metabolism, Neurosurgery, NGF, Polymers - pharmacology, polypyrrole, Pyrroles - pharmacology, Rats, Rats, Sprague-Dawley, RNA, Messenger - genetics, RNA, Messenger - metabolism, Schwann cell, Schwann Cells - cytology, Schwann Cells - drug effects, Schwann Cells - ultrastructure, Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases, Technology. Biomaterials. Equipments, viability