Details

Autor(en) / Beteiligte

• Kuhn, Graciele
• Marangoni, Marluci
• Freire, Denise M.G
• Soares, Valéria Ferreira
• de Godoy, Mateus Gomes
• de Castro, Aline Machado
• Luccio, Marco Di
• Treichel, Helen
• Mazutti, Marcio A
• Oliveira, Débora
• Oliveira, J. Vladimir

Titel

Esterification activities of non-commercial lipases after pre-treatment in pressurized propane

Ist Teil von

• Journal of chemical technology and biotechnology (1986), 2010-06, Vol.85 (6), p.839-844

Ort / Verlag

Chichester, UK: John Wiley & Sons, Ltd

Erscheinungsjahr

2010

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• BACKGROUND: The objective of this work was to assess the influence of propane pre-treatment on the esterification activities of two non-commercial lipases from Penicillium simplicissimum and Aspergillus parasiticus in the lyophilized and immobilized forms, evaluating the effect of pressure, exposure time and depressurization rate on the lipase activity by a full 2³ experimental design.RESULTS: For both lyophilized and immobilized lipases from all sources evaluated, enhancement in residual activities after incubation in pressurized propane was observed, under several experimental conditions. The highest increment (427%) occurred with the lyophilized enzyme from P. simplicissimum was maintained pressurized at 30 bar for 1 h and then depressurized at the fastest rate (20 bar min⁻¹).CONCLUSION: The enzyme activity changes significantly depending on the enzyme source, its presentation form, and the experimental pre-treatment conditions investigated, such as exposure time, depressurization rate and pressure. The results obtained here can contribute as a basis for the selection of appropriate operational conditions, so that these catalysts can be applied in biotransformation reactions. Copyright