Proteomics (Weinheim), 2011-02, Vol.11 (3), p.495-500
2011
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Details
- Autor(en) / Beteiligte
- Titel
- Analysis of the human pituitary proteome by data independent label-free liquid chromatography tandem mass spectrometry
- Ist Teil von
- Proteomics (Weinheim), 2011-02, Vol.11 (3), p.495-500
- Ort / Verlag
- Weinheim: Wiley-VCH Verlag
- Erscheinungsjahr
- 2011
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Studies of pituitary-related disorders would be facilitated by enhanced knowledge of the pituitary proteome. To construct a data set of human pituitary proteins, separate protein extracts were prepared from 15 post-mortem pituitaries and analyzed by data independent label-free nanoflow liquid chromatography mass spectrometry (nLC-MSE). The detected mass/time features were aligned and quantified using the Rosetta Elucidator® system and annotated using results from ProteinLynx Global Server. The resulting data set comprised 1007 unique proteins, with stringent identification by a minimum of two distinct peptides. These proteins consisted predominantly of enzymes, transporters, transcription/translation factors, cell structure and secreted proteins.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1615-9853, 1615-9861eISSN: 1615-9861DOI: 10.1002/pmic.201000496Titel-ID: cdi_proquest_miscellaneous_883015250
- Format
- –
- Schlagworte
- Analytical, structural and metabolic biochemistry, Animal proteomics, Autopsy, Biological and medical sciences, Biomarkers - metabolism, Chromatography, Chromatography, Liquid, Fundamental and applied biological sciences. Psychology, Humans, Mass spectrometry, Miscellaneous, Neuropeptides - analysis, Neuropeptides - metabolism, Pituitary, Pituitary Gland - cytology, Pituitary Gland - metabolism, Protein biomarker, Proteins, Proteome - analysis, Proteome - metabolism, Proteomics, Proteomics methods, Tandem Mass Spectrometry