Details

Autor(en) / Beteiligte

• Hulstijn, Maarten
• Barros, Lucas de Andrade
• Neves, Renata Heisler
• de Moura, Egberto Gaspar
• Machado-Silva, José Roberto

Titel

Parasitological and morphological study of Schistosoma mansoni and diabetes mellitus in mice

Ist Teil von

• Experimental parasitology, 2011-09, Vol.129 (1), p.42-47

Ort / Verlag

Amsterdam: Elsevier Inc

Erscheinungsjahr

2011

Quelle

MEDLINE

Beschreibungen/Notizen

• Streptozotocin-induced diabetic mice were infected with Schistosoma mansoni. Mice that did not receive the drug remained as controls. Susceptibility to infection was evaluated by quantifying fecal egg excretion 7–9 weeks post-infection. Mice were euthanized the day after the last fecal examination was performed. Adult worms were recovered from the portal system and mesenteric veins, whereas liver and intestine were removed for enumeration of egg load. [Display omitted] ► The average load of adult worms was lower in diabetic mice than in non-diabetic mice. ► No morphological divergences in either tegument or reproductive organs were observed between above groups. ► Morphometric analysis did not show differences between worms recovered from diabetic or non-diabetic mice. ► Diabetic mice displayed higher tissue immature eggs than non-diabetic mice. ► Diabetic mice displayed lower fecal egg count than non-diabetic mice. Schistosomes are blood-dwelling flukes which are highly dependent on the host metabolism. The aim of this study was to investigate possible relationship between streptozotocin-induced diabetes and the outcome of acute murine schistosomiasis mansoni. Male and female SW mice were treated by a single intraperitoneally injected dose of streptozotocin (180mg/kg). Seven days after induction, both control and diabetic animals were infected with 70 Schistosoma mansoni cercariae (BH strain). Diabetics and their controls were weighed 45days after birth and for the last time prior to killing. Susceptibility to infection was evaluated twice a week by quantifying fecal egg excretion 7–9weeks post-infection by the Kato–Katz’ thick smear method. Mice were euthanized the day after the last fecal examination was performed. Adult worms were recovered from the portal system and mesenteric veins, whereas liver and intestine were removed for enumeration of egg load. No differences in worm length or in measurements of the reproductive organs, tegument, and suckers were detected. Also oviposition was unaffected as the total number of eggs per female worm from the liver, the small and the large intestine was the same in both groups. An oogram evaluation revealed a lower percentage of mature (23.0% vs. 40.7%) and a higher percentage of immature (69.1% vs. 51.7%) eggs in the small intestine of the diabetic mice. We suggest that principally a hampered egg passage through the intestine tissue caused this reduction and that probably both the eggs and the impaired host response play a role.